Document Detail


Microporous cell-laden hydrogels for engineered tissue constructs.
MedLine Citation:
PMID:  20091766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this article, we describe an approach to generate microporous cell-laden hydrogels for fabricating biomimetic tissue engineered constructs. Micropores at different length scales were fabricated in cell-laden hydrogels by micromolding fluidic channels and leaching sucrose crystals. Microengineered channels were created within cell-laden hydrogel precursors containing agarose solution mixed with sucrose crystals. The rapid cooling of the agarose solution was used to gel the solution and form micropores in place of the sucrose crystals. The sucrose leaching process generated homogeneously distributed micropores within the gels, while enabling the direct immobilization of cells within the gels. We also characterized the physical, mechanical, and biological properties (i.e., microporosity, diffusivity, and cell viability) of cell-laden agarose gels as a function of engineered porosity. The microporosity was controlled from 0% to 40% and the diffusivity of molecules in the porous agarose gels increased as compared to controls. Furthermore, the viability of human hepatic carcinoma cells that were cultured in microporous agarose gels corresponded to the diffusion profile generated away from the microchannels. Based on their enhanced diffusive properties, microporous cell-laden hydrogels containing a microengineered fluidic channel can be a useful tool for generating tissue structures for regenerative medicine and drug discovery applications.
Authors:
Jae Hong Park; Bong Geun Chung; Won Gu Lee; Jinseok Kim; Mark D Brigham; Jaesool Shim; Seunghwan Lee; Chang Mo Hwang; Naside Gozde Durmus; Utkan Demirci; Ali Khademhosseini
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  106     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-06-07     Revised Date:  2011-05-13    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  138-48     Citation Subset:  IM    
Affiliation:
Department of Medicine, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Rm 265, Cambridge, Massachusetts 02139, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Cell Line, Tumor
Cold Temperature
Culture Media / chemistry
Humans
Hydrogels*
Sepharose / chemistry
Sucrose / chemistry
Tissue Engineering / methods*
Grant Support
ID/Acronym/Agency:
DE19024/DE/NIDCR NIH HHS; EB007249/EB/NIBIB NIH HHS; HL092836/HL/NHLBI NIH HHS; R01 HL092836-01A1/HL/NHLBI NIH HHS; R01 HL092836-02/HL/NHLBI NIH HHS; R01 HL092836-02S1/HL/NHLBI NIH HHS; R21 EB007249-01/EB/NIBIB NIH HHS; R21 EB007249-02/EB/NIBIB NIH HHS; RL1 DE019024-01/DE/NIDCR NIH HHS; RL1 DE019024-02/DE/NIDCR NIH HHS; RL1 DE019024-03/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Hydrogels; 57-50-1/Sucrose; 9012-36-6/Sepharose
Comments/Corrections

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