Document Detail

Micropipette aspiration of living cells.
MedLine Citation:
PMID:  10609514     Owner:  NLM     Status:  MEDLINE    
The mechanical behavior of living cells is studied with micropipette suction in which the surface of a cell is aspirated into a small glass tube while tracking the leading edge of its surface. Such edges can be tracked in a light microscope to an accuracy of +/-25 nm and suction pressures as small as 0.1-0.2 pN/microm2 can be imposed on the cell. Both soft cells, such as neutrophils and red cells, and more rigid cells, such as chondrocytes and endothelial cells, are studied with this technique. Interpretation of the measurements with basic continuum models leads to values for a cell's elastic and viscous properties. In particular, neutrophils are found to behave as a liquid drop with a cortical (surface) tension of about 30 pN/microm and a viscosity on the order of 100 Pa s. On the other hand, chondrocytes and endothelial cells behave as solids with an elastic modulus of the order of 500 pN/microm2 (0.5 kPa).
R M Hochmuth
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Journal of biomechanics     Volume:  33     ISSN:  0021-9290     ISO Abbreviation:  J Biomech     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-07     Completed Date:  2000-02-07     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0157375     Medline TA:  J Biomech     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15-22     Citation Subset:  IM; S    
Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708-0300, USA.
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MeSH Terms
Cell Physiological Phenomena*
Chondrocytes / physiology,  ultrastructure
Endothelium / cytology,  physiology
Erythrocytes / physiology,  ultrastructure
Models, Biological
Neutrophils / physiology,  ultrastructure
Suction / instrumentation,  methods
Grant Support

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