Document Detail


Micropipette aspiration of human erythrocytes induces echinocytes via membrane phospholipid translocation.
MedLine Citation:
PMID:  9138589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When a discocytic erythrocyte (RBC) was partially aspirated into a 1.5-microns glass pipette with a high negative aspiration pressure (delta P = -3.9 kPa), held in the pipette for 30 s (holding time, th), and then released, it underwent a discocyte-echinocyte shape transformation. The degree of shape transformation increased with an increase in th. The echinocytes recovered spontaneously to discocytes in approximately 10 min, and there was no significant difference in recovery time at 20.9 degrees C, 29.5 degrees C, and 37.4 degrees C, respectively. At 11 degrees C the recovery time was significantly elevated to 40.1 +/- 6.7 min. At 20.9 degrees C the shape recovery time varied directly with the isotropic RBC tension induced by the pipetting. Sodium orthovanadate (vanadate, 200 microM), which inhibits the phospholipid translocase, blocks the shape recovery. Chlorpromazine (CP, 25 microM) reversed the pipette-induced echinocytic shape to discocytic in < 2 min, and the RBC became a spherostomatocyte-II after another 30 min. It was hypothesized that the increase in cytosolic pressure during the pipette aspiration induced an isotropic tension in the RBC membrane followed by a net inside-to-outside membrane lipid translocation. After a sudden release of the aspiration pressure the cytosolic pressure and the membrane tension normalized immediately, but the translocated phospholipids remained temporarily "trapped" in the outer layer, causing an area excess and hence the echinocytic shape. The phospholipid translocase activity, when not inhibited by vanadate, caused a gradual return of the translocated phospholipids to the inner layer, and the RBC shape recovered with time.
Authors:
G M Artmann; K L Sung; T Horn; D Whittemore; G Norwich; S Chien
Related Documents :
24084759 - Direct measurement of osmotic pressure via adaptive confinement of quasi hard disc coll...
6687589 - High-frequency oscillations via the pleural surface: an alternative mode of ventilation?
8002489 - Accurate method to study static volume-pressure relationships in small fetal and neonat...
7767549 - Surfactant protein-a deficiency in a primate model of bronchopulmonary dysplasia.
9142589 - Surfactant nebulisation: safety, efficiency and influence on surface lowering propertie...
8148579 - Randomized placebo-controlled study on the characteristics and duration of action of su...
11378599 - Effect of a catecholamine-induced increase in cardiac output on extravascular lung water.
9272189 - An abnormal relationship between blood pressure and pulse rate in amyotrophic lateral s...
3593069 - Increased pulse pressure in patients with arteriosclerosis obliterans of the lower limbs.
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  72     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-05-05     Completed Date:  1997-05-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1434-41     Citation Subset:  IM    
Affiliation:
Department of Applied Cell Biophysics, FH Aachen, Jülich, Germany. artmann@fh-aachen.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blood Specimen Collection / instrumentation,  methods
Carrier Proteins / antagonists & inhibitors,  blood
Chlorpromazine / pharmacology
Cytosol / physiology
Erythrocyte Membrane / physiology,  ultrastructure*
Erythrocytes / cytology*,  drug effects,  physiology
Humans
Kinetics
Membrane Lipids / blood*
Membrane Proteins / antagonists & inhibitors,  blood
Phospholipid Transfer Proteins*
Phospholipids / blood*
Pressure
Regression Analysis
Time Factors
Vanadates / pharmacology
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Lipids; 0/Membrane Proteins; 0/Phospholipid Transfer Proteins; 0/Phospholipids; 0/Vanadates; 50-53-3/Chlorpromazine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Modification of calcite crystal growth by abalone shell proteins: an atomic force microscope study.
Next Document:  Odorant-induced currents in intact patches from rat olfactory receptor neurons: theory and experimen...