| Micropatterning contractile C2C12 myotubes embedded in a fibrin gel. | |
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MedLine Citation:
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PMID: 20014142 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Contractile C(2)C(12) myotube line patterns embedded in a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. The C(2)C(12) myotube/fibrin gel system was prepared by transferring a myotube monolayer from a glass substrate to a fibrin gel while retaining the original line patterns of myotubes. To endow the myotubes with contractile activity, a series of electrical pulses was applied through a pair of carbon electrodes placed at either side of a fibrin gel separately. The frequency and magnitude of myotube contraction were functions of the pulse frequency and duration, respectively. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (1 week) than myotubes adhered on a conventional culture dish. |
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Authors:
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Kuniaki Nagamine; Takeaki Kawashima; Takeshi Ishibashi; Hirokazu Kaji; Makoto Kanzaki; Matsuhiko Nishizawa |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 105 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-02 Completed Date: 2010-05-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1161-7 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley Periodicals, Inc. |
Affiliation:
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Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Sendai, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electric Stimulation Fibrin / chemistry* Mice Microtechnology Muscle Contraction / physiology* Muscle Fibers, Skeletal / cytology, physiology*, ultrastructure Tissue Culture Techniques / methods* Tissue Scaffolds / chemistry* |
| Chemical | |
Reg. No./Substance:
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9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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