Document Detail


Micropatterning of cells using modulated magnetic fields.
MedLine Citation:
PMID:  15667156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new technique of cell micropatterning was presented. Mouse osteoblast cells (MC3T3-E1) were seeded on a substrate whose surface was exposed to a periodically modulated magnetic field (a line pattern with a 200- or 600-microm pitch) produced by a field modulator inserted into a homogeneous magnetic field of 1 T generated by an electromagnet. The cells were trapped consistent with the line profile of the modulated field. The trapping efficiency was enhanced by adding Mn(II)EDTA (paramagnetic) to the cultivation medium. The cells were subsequently incubated in the magnetic field. The same technique was applied to whole blood to pattern red blood cells.
Authors:
Tsunehisa Kimura; Yukiko Sato; Fumiko Kimura; Masakazu Iwasaka; Shoogo Ueno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  21     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-25     Completed Date:  2006-05-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  830-2     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan. kimura-tsunehisa@c.metro-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cells, Immobilized*
Erythrocytes
Humans
Magnetics
Mice

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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