Document Detail


Micropatterning bacterial suspensions using aqueous two phase systems.
MedLine Citation:
PMID:  20838666     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Using an aqueous two-phase system comprised of dextran and polyethylene glycol, this article describes the stable spatial patterning of sub-microlitre droplets of bacterial suspensions. Microdroplets of different types of bacterial populations are positioned and maintained adjacent to each other without significant dispersion even though the bacteria are in suspension and not surface bound. Small molecules, in contrast, diffuse relatively freely between the two aqueous phases. The usefulness of these capabilities is demonstrated by generating a small array of suspensions containing different Escherichia coli strains engineered to respond fluorescently or luminescently to different chemical stimuli. When a chemical stimulus is presented, this droplet array produces a pattern of bacterial "illumination" that reflects the type of chemical to which the array was exposed.
Authors:
Toshiyuki Yaguchi; Siseon Lee; Woon Sun Choi; Dasol Kim; Taesung Kim; Robert J Mitchell; Shuichi Takayama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-13
Journal Detail:
Title:  The Analyst     Volume:  135     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  2848-52     Citation Subset:  IM    
Affiliation:
School of Nano-biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Banyeon-ri 100, Ulsan, 689-798, Republic of Korea.
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