Document Detail

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells.
MedLine Citation:
PMID:  17508289     Owner:  NLM     Status:  MEDLINE    
In this paper the easy and reliable preparation of precise micropatterns on PDMS surfaces is described and the growth of HEK 293 cells on those patterns during culture over several days is examined. The first patterning approach described is based on soft-lithography and polyelectrolyte multilayer deposition. Two different soft-lithographic techniques are employed for creating surface patterns of PAH, PSS, untreated and oxidized PDMS. The growth behavior of HEK 293 cells is investigated on all the dual combinations of the four surfaces, and decreasing preference of the cells for the surfaces in the order PAH (-NH2)>ox-PDMS (-OH)>>PSS (-SO3-)>PDMS (-CH3) is revealed. As the second patterning approach a method is introduced, which allows the deposition of gel droplets in a microarray format utilizing differences in the surface wettability. This concept is new and expected to be very useful for various applications. Finally, a speculative explanation for the different cell spreading behavior is provided considering the interplay between individual cell-surface interactions and a permanent cell tractional force.
R M Johann; Ch Baiotto; Ph Renaud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical microdevices     Volume:  9     ISSN:  1387-2176     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-09     Completed Date:  2007-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  475-85     Citation Subset:  IM    
Fraunhofer-Institute for Biomedical Engineering, IBMT, D-66386, St. Ingbert, Germany.
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MeSH Terms
Cell Adhesion
Cell Proliferation*
Cells, Cultured
Dimethylpolysiloxanes / chemistry*
Silicones / chemistry*
Surface Properties
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Gels; 0/Silicones; 63148-62-9/baysilon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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