Document Detail

Micropatterned hot-embossed polymeric surfaces influence cell proliferation and alignment.
MedLine Citation:
PMID:  18314900     Owner:  NLM     Status:  MEDLINE    
Micropatterning is a powerful technique to custom-make and precisely control the surface topography of materials, which is determinant for a better interaction with cells. A modification of conventional micropatterning is proposed here to fabricate textured film from stiff and sticky polymers such as poly(lactide(s)-co-glycolide(s)) (PLGA) without the use of supports or solvents. Micropatterned PLGA films with square pits varying in height and channels varying in width were made to study the influence of these topographical parameters on human fibroblasts proliferation, morphology, and alignment. With increasing the square pit height, the cell attachment efficiency increased. After 10 days of culture the micropatterned films supported a significantly higher cell proliferation than smooth films. In particular, cell growth was highly stimulated in 150-mum-wide channels. Fibroblasts were spread with a typical spindle shape in all the films. Cell spreading increased with increasing the textured dimensions. A random cell organization was found for smooth and for square pit samples, and a high alignment was observed along the 150-mum-wide channels. Smaller and bigger channels did not support substantial cell growth, suggesting a possible "recognition" mechanism of the cells for optimal organization. These findings could be useful in tissue engineering applications where higher proliferation rates and eventual random or unidimensional alignments of cells are desirable.
Lorenzo Moroni; Luke P Lee
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  88     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  644-53     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley Periodicals, Inc.
Department of Bioengineering, University of California Berkeley, 459 Evans Hall No. 1762, UC Berkeley, Berkeley, California 94720-1762, USA.
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MeSH Terms
Cell Line
Cell Movement*
Cell Proliferation
Fibroblasts / cytology
Hot Temperature*
Lactic Acid / chemistry*
Microscopy, Electron, Scanning
Polyglycolic Acid / chemistry*
Surface Properties
Reg. No./Substance:
0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

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