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Microparticulate polyelectrolyte complexes for gentamicin transport across intestinal epithelia.
MedLine Citation:
PMID:  20726809     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Polysaccharide microparticles for the oral administration of gentamicin were designed in order to obtain an increased drug absorption by means of microparticle transport across the intestinal epithelia. Alginate/chitosan microparticles with a size of ~2 μm were developed by spray-drying a water solution containing the drug complexed with the polyanionic alginate and subsequent alginate cross-linking process by calcium ions and chitosan. The pre-formulation study, performed by changing the concentration of both cross-linkers, led to the selection of the most suitable formulation which was assayed for its capacity to be translocated across intestinal epithelia, via both M cells contained in Follicle Associated Epithelium (FAE) of Peyer's patches and enterocytes of the mucosal epithelium. An ex vivo perfusion technique of rabbit and rat intestinal tissues containing Peyer's patches combined with an in vitro method by using Caco-2 cell monolayers demonstrated the microparticulate carrier ability to be taken up by both M cells and enterocytes. However, only the endocytosis by M cells appeared to provide the microparticle transport from the epithelium toward deeper sub-epithelial regions.
Authors:
Valentina Iannuccelli; Monica Montanari; Davide Bertelli; Federica Pellati; Gilberto Coppi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-21
Journal Detail:
Title:  Drug delivery     Volume:  18     ISSN:  1521-0464     ISO Abbreviation:  Drug Deliv     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9417471     Medline TA:  Drug Deliv     Country:  England    
Other Details:
Languages:  eng     Pagination:  26-37     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Italy. valentina.iannuccelli@unimore.it
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