| Microparticle formation after exposure of blood to activated endothelium under flow. | |
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MedLine Citation:
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PMID: 20653016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Increased numbers of circulating microparticles (MPs) are indicative of poor clinical outcome in a number of inflammatory disorders, including atherosclerosis. Platelets and megakaryocytes are a major source of MP and are identified by presence of CD42b on the MP surface. MP shed from activated platelets can be identified by presence of P-selectin (CD62P). Tissue factor (TF) is the principal initiator of blood coagulation and its activity has been identified in MPs derived from patient plasma, which may contribute to thrombosis. Here, we have investigated by flow cytometry the expression of TF and CD62P on MP after exposure of diluted whole blood to TNF-activated endothelial cells (EC) both under static conditions and in our newly established model of flow. MPs were significantly increased in blood subjected to flow and this was further enhanced after exposure of blood to TNF-activated EC. MP surface expression of CD62P or TF was upregulated following exposure to TNF-activated EC under flow compared with flow with nonactivated EC or after static coculture with and without prior EC activation. These data strongly suggest that interactions of blood with inflamed EC can modulate production of CD62P and TF bearing MP under flow conditions, and thus may contribute to a prothrombotic environment. |
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Authors:
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Marion G Macey; Sabine I Wolf; Charlotte Lawson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytometry. Part A : the journal of the International Society for Analytical Cytology Volume: 77 ISSN: 1552-4930 ISO Abbreviation: Cytometry A Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235694 Medline TA: Cytometry A Country: United States |
Other Details:
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Languages: eng Pagination: 761-8 Citation Subset: IM |
Affiliation:
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Department of Haematology, The Royal London Hospital, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Cells / cytology, metabolism* Blood Platelets / cytology, metabolism Cell-Derived Microparticles / metabolism* Coculture Techniques Endothelial Cells / cytology, metabolism Endothelium / metabolism* Female Flow Cytometry Hemorheology* Humans Leukocytes / cytology, metabolism Male P-Selectin / metabolism Thromboplastin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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PG/05/114//British Heart Foundation |
| Chemical | |
Reg. No./Substance:
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0/P-Selectin; 9035-58-9/Thromboplastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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