Document Detail


Microparticle-enhanced nephelometric immunoassay of human plasminogen.
MedLine Citation:
PMID:  8852360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free antibodies of anti-plasminogen rabbit antiserum. This immunoassay was sensitive (detection limit in reaction mixture, 34 micrograms/L) and could be performed in 500-fold diluted human plasma, excluding any interference or sample pretreatment. It allowed the quantitation of plasminogen on a large range of concentrations (17-550 mg/L), with a security in antigen excess reaching 1,100 mg/L, with accuracy (linear recovery in dilution-overloading assay and correlation with conventional immunonephelometry), and precision (within- and between-run coefficients of variation lower than 8%). A normal reference range from 54 to 148 mg/L (mean +/- 2 SD) was calculated from plasminogen concentration in plasma from 130 adults. Easy to perform (no washing or phase separation) and rapid (two steps of 30 minutes then 1 hour of incubation at room temperature), this microparticle-enhanced nephelometric immunoassay could be an interesting alternative method for human plasminogen quantitation.
Authors:
E Marchal; P Montagne; M L Cuillière; M C Béné; G Faure
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  10     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-06     Completed Date:  1996-12-06     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  85-90     Citation Subset:  IM    
Affiliation:
Immunology Laboratory, Faculty of Medicine, Nancy, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibody Specificity
Female
Humans
Immunoassay / methods
Male
Middle Aged
Nephelometry and Turbidimetry / methods
Particle Size
Plasminogen / analysis*,  immunology
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
9001-91-6/Plasminogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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