Document Detail


Microorifice-based high-yield cell fusion on microfluidic chip: electrofusion of selected pairs and fusant viability.
MedLine Citation:
PMID:  20142145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microorifice-based fusion makes use of electric field constriction to assure high-yield one-to-one fusion of selected cell pairs. The aim of this paper is to verify feasibility of high-yield cell fusion on a microfluidic chip. This paper also examines viability of the fusant created on the chip. We fabricated a microfluidic chip to fuse selected cell pairs and to study postfusion behavior. We used a self-forming meniscus-based fabrication process to create microorifice with a diameter of 2-10 microm on the vertical walls in a microfluidic channel. When 1 MHz was applied to electrodes located on both sides of the microorifice, dielectrophoretic force attracted the cells toward microorifice to form a cell pair. Once the cells get into contact, fusion pulse was applied. Real time imaging of cells during fusion and cytoplasmic dye transfer between cells indicated success of cell fusion. We found that when high frequency voltage for dielectrophoresis was swept from 1 MHz to 10 kHz in 100 micros, cell fusion was initiated. The effective electric field strength was 0.1-0.2 kV/cm. We analyzed viability by imaging fusant going into cell division phase after 48 h of incubation. We conclude that fabricated microfluidic chip is suitable for high-yield one-to-one fusion and creation of viable fusants. This technology should be a useful tool to study fusion phenomena and viability of fusants, as it allows imaging of the cells during and after the fusion.
Authors:
M Gel; S Suzuki; Y Kimura; O Kurosawa; B Techaumnat; H Oana; M Washizu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  IEEE transactions on nanobioscience     Volume:  8     ISSN:  1558-2639     ISO Abbreviation:  IEEE Trans Nanobioscience     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-02-09     Completed Date:  2010-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101152869     Medline TA:  IEEE Trans Nanobioscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  300-5     Citation Subset:  IM    
Affiliation:
Department of Mechanical Engineering, University of Tokyo, Tokyo 113-8656, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomedical Engineering
Cell Fusion / instrumentation*,  methods
Cell Line
Cell Survival
Electricity
Equipment Design
Humans
Jurkat Cells
Mice
Microfluidic Analytical Techniques / instrumentation*,  methods
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Microscopy, Phase-Contrast

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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