| Micronucleus frequency in sheep lymphocytes after in vitro exposure to fungicide tolylfluanid. | |
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MedLine Citation:
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PMID: 20803363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fungicide tolylfluanid (N -dichlorofluoromethylthio-N', N -dimethyl -N -p -tolylsulfamide), was investigated by cytokinesis-block micronucleus assay. Tolylfluanid at the lowest concentration (1 x 10(- 6)mol L(- 1))did not influence significantly the frequency of micronuclei in sheep lymphocyte cultures in comparison with control (32.33 +/- 3.51/1000 binucleated cells versus 30.33 +/- 2.82/1000 binucleated cells in dimethylsulfoxide (DMSO) control, P = 0.44). Higher tolylfluanid concentrations (1 x 10(- 4) and, 1 x 10(- 5) mol L(- 1)) resulted in a significant dose-dependent increase in the number of micronuclei in comparison with control (74.00 +/- 13.00/1000 binucleated cells and 52.67 +/- 10.12/1000 binucleated cells versus 30.33 +/- 2. 82/1000 binucleated cells in DMSO control, P = 0.005 and 0.02, respectively, ANOVA followed by Tukey test P < 0.05). Many of the treated cells also possessed multiple micronuclei. Tolylfluanid did not affect the nuclear division index at all treatment concentrations. Our in vitro results thus demonstrate that tolylfluanid had a significant genotoxic effect at only the highest concentration tested. |
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Authors:
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Irena Sutiaková; Natália Kovalkovicová; Jaroslav Legáth; Václav Sutiak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes Volume: 45 ISSN: 1532-4109 ISO Abbreviation: J Environ Sci Health B Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-30 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7607167 Medline TA: J Environ Sci Health B Country: England |
Other Details:
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Languages: eng Pagination: 606-11 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Presov, Presov, Slovakia. sutiakova@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aniline Compounds
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toxicity* Animals Cell Division / drug effects Female Fungicides, Industrial / toxicity* Lymphocytes / cytology, drug effects* Micronuclei, Chromosome-Defective / drug effects* Micronucleus Tests Mutagens / toxicity* Sheep Sulfonamides / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Fungicides, Industrial; 0/Mutagens; 0/Sulfonamides; 731-27-1/tolyfluanid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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