Document Detail

Micronucleus frequency in sheep lymphocytes after in vitro exposure to fungicide tolylfluanid.
MedLine Citation:
PMID:  20803363     Owner:  NLM     Status:  MEDLINE    
The fungicide tolylfluanid (N -dichlorofluoromethylthio-N', N -dimethyl -N -p -tolylsulfamide), was investigated by cytokinesis-block micronucleus assay. Tolylfluanid at the lowest concentration (1 x 10(- 6)mol L(- 1))did not influence significantly the frequency of micronuclei in sheep lymphocyte cultures in comparison with control (32.33 +/- 3.51/1000 binucleated cells versus 30.33 +/- 2.82/1000 binucleated cells in dimethylsulfoxide (DMSO) control, P = 0.44). Higher tolylfluanid concentrations (1 x 10(- 4) and, 1 x 10(- 5) mol L(- 1)) resulted in a significant dose-dependent increase in the number of micronuclei in comparison with control (74.00 +/- 13.00/1000 binucleated cells and 52.67 +/- 10.12/1000 binucleated cells versus 30.33 +/- 2. 82/1000 binucleated cells in DMSO control, P = 0.005 and 0.02, respectively, ANOVA followed by Tukey test P < 0.05). Many of the treated cells also possessed multiple micronuclei. Tolylfluanid did not affect the nuclear division index at all treatment concentrations. Our in vitro results thus demonstrate that tolylfluanid had a significant genotoxic effect at only the highest concentration tested.
Irena Sutiaková; Natália Kovalkovicová; Jaroslav Legáth; Václav Sutiak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes     Volume:  45     ISSN:  1532-4109     ISO Abbreviation:  J Environ Sci Health B     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7607167     Medline TA:  J Environ Sci Health B     Country:  England    
Other Details:
Languages:  eng     Pagination:  606-11     Citation Subset:  IM    
Department of Biology, University of Presov, Presov, Slovakia.
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MeSH Terms
Aniline Compounds / toxicity*
Cell Division / drug effects
Fungicides, Industrial / toxicity*
Lymphocytes / cytology,  drug effects*
Micronuclei, Chromosome-Defective / drug effects*
Micronucleus Tests
Mutagens / toxicity*
Sulfonamides / toxicity*
Reg. No./Substance:
0/Aniline Compounds; 0/Fungicides, Industrial; 0/Mutagens; 0/Sulfonamides; 731-27-1/tolyfluanid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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