Document Detail

Micronuclei in mouse skin cells following in vivo exposure to benzo[a]pyrene, 7,12-dimethylbenz[a]anthracene, chrysene, pyrene and urethane.
MedLine Citation:
PMID:  1902414     Owner:  NLM     Status:  MEDLINE    
Detection of micronuclei (MN) in skin cells from HRA/Skh hairless mice treated with chemical or physical agents may prove informative in qualitative and quantitative studies of skin carcinogenesis. MN induction and cell survival were estimated in cytokinesis-blocked keratinocytes, cultured for 4 days in vitro, after a single topical dose of various organic compounds. Treatment with 2.56 micrograms (10 nmol) 7,12-dimethylbenz[a] anthracene (DMBA) resulted in maximal MN induction in cells removed from skin 12-24 hr after topical administration (79-88 MN/1,000 cells compared with 10-16 MN/1,000 cells in acetone-treated controls). Even in cells removed only 1 hr after DMBA treatment, a significant increase in MN was evident. However, to allow sufficient time for metabolic activation, a sampling time for of 24 hr was adopted for all test substances. Dose-dependent increases in MN were observed with DMBA, benzo[a]pyrene, chrysene, and urethane. Increased numbers of micronucleated cells were detected at the lowest doses administered in the present study (0.128, 0.5, 50, and 50 micrograms, respectively). Although reduced cell recovery occurred following exposure of mice to acetone, pyrene, and other chemicals, there was no evidence that cytotoxicity contributed to MN scored in keratinocytes. Moreover, the probable noncarcinogen, pyrene, failed to induce MN at doses from 2.5 micrograms to 2.5 mg/mouse. These results show that it is possible to assess chemical exposure in skin by measuring cell survival and skin genotoxicity by measuring MN induction in cultured keratinocytes. The available data suggest that MN induction may be a useful indicator of the carcinogenic potential of chemicals applied to the skin.
S L He; R Baker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Environmental and molecular mutagenesis     Volume:  17     ISSN:  0893-6692     ISO Abbreviation:  Environ. Mol. Mutagen.     Publication Date:  1991  
Date Detail:
Created Date:  1991-05-31     Completed Date:  1991-05-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8800109     Medline TA:  Environ Mol Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  163-8     Citation Subset:  IM    
Guangxi Medical College, Nanning, Guangxi, Peoples' Republic of China.
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MeSH Terms
9,10-Dimethyl-1,2-benzanthracene / toxicity
Benzo(a)pyrene / toxicity
Carcinogens / toxicity*
Chi-Square Distribution
Chrysenes / toxicity
Mice, Hairless
Micronucleus Tests / methods*
Pyrenes / toxicity
Skin / cytology,  drug effects*
Time Factors
Urethane / toxicity
Reg. No./Substance:
0/Carcinogens; 0/Chrysenes; 0/Pyrenes; 129-00-0/pyrene; 218-01-9/chrysene; 50-32-8/Benzo(a)pyrene; 51-79-6/Urethane; 57-97-6/9,10-Dimethyl-1,2-benzanthracene

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