Document Detail

Micronuclei in exfoliated human cells as a tool for studies in cancer risk and cancer intervention.
MedLine Citation:
PMID:  6713365     Owner:  NLM     Status:  MEDLINE    
The use of the micronucleus test on exfoliated cells as an approach to identify genotoxic damage in human tissues which are targets for organ-specific carcinogens and from which carcinomas will develop, is described. Chromosomal damage by carcinogens to dividing basal cells of the epithelium results in the production of micronuclei in the daughter cells which migrate up through the epithelium and are exfoliated. Exfoliated cells can be readily obtained from several tissues, including the oral buccal mucosa (scrapings of oral cells), bronchi (sputum), urinary bladder and ureter (centrifugation of urine), cervix (smears) and esophagus (imprints from biopsies). The micronucleus test on exfoliated cells has been successfully used to: (1) recognize population groups at an elevated risk for cancer of the oral cavity or urinary bladder; (2) estimate synergistic or additive effects of carcinogen exposure (cigarette smokers plus drinkers of alcoholic beverages); (3) pinpoint the site within an organ from which most carcinomas will develop (oral cancers among 'inverted' smokers in the Philippines). The possibility that this assay may also serve as a rapid monitor for chemopreventive agents is suggested by a preliminary trial on the effect of vitamin A/beta--carotene dietary supplementation among 33 betel quid chewers in the Philippines. These individuals received sealed capsules of retinol (100,000 IU/week) and beta-carotene (300,000 IU/week) for a 3-month period. At the end of this time, the frequencies of micronucleated buccal mucosa cells were reduced from an average of 4.2% to 1.4%. No changes were observed in micronucleus frequencies among 11 betel quid chewers not receiving vitamin pills. Non- chewers of betel quid in this population had a micronucleus frequency of 0.5%.
H F Stich; M P Rosin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  22     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-06-20     Completed Date:  1984-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  241-53     Citation Subset:  IM    
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MeSH Terms
Carcinogens / toxicity*
Carotenoids / therapeutic use
Chromosome Aberrations
Continental Population Groups
Ethnic Groups
Mouth Mucosa / drug effects,  ultrastructure*
Mouth Neoplasms / drug therapy,  prevention & control*
Mutagenicity Tests*
Plants, Medicinal
Plants, Toxic
Vitamin A / therapeutic use
beta Carotene
Reg. No./Substance:
0/Carcinogens; 11103-57-4/Vitamin A; 36-88-4/Carotenoids; 7235-40-7/beta Carotene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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