Document Detail


Micronuclei and 3AB index in X-irradiated human lymphocytes in G0 and G1 phases.
MedLine Citation:
PMID:  7526188     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We applied the cytokines-block micronucleus assay to observe the radiobiological response of human lymphocytes after X-ray treatment in the G0 and G1 phases. In addition, we used 3-aminobenzamide (3AB) to measure the 3AB index in the two phases. The experimental results show that at 2 Gy the MN yield and the 3AB index are dependent on the cell phase and show considerable inter-individual variability. The radiation-induced MN frequency obtained for 33 subjects is 0.470 +/- 0.063 for the G0 phase and 0.689 +/- 0.139 for the G1 phase; the 3AB index values are 0.326 +/- 0.144 and 0.067 +/- 0.058 for G0 and G1 phases, respectively. At the individual level, the 3AB index for the G1 phase correlates inversely with the cytogenetic effects observed in that phase. We discuss the possibility of applying the MN test combined with the 3AB index to lymphocytes at different phases to study the individual response to radiation (individual radiosensitivity).
Authors:
C Catena; P Villani; D Conti; E Righi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  311     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1994-12-16     Completed Date:  1994-12-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  231-7     Citation Subset:  IM    
Affiliation:
AMB-EFF, ENEA CRE Casaccia, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Benzamides / toxicity*
DNA Repair / drug effects
Dose-Response Relationship, Radiation
G0 Phase / drug effects,  radiation effects
G1 Phase / drug effects,  radiation effects
Genetic Variation
Humans
Interphase / drug effects,  radiation effects*
Lymphocytes / drug effects,  radiation effects*
Micronucleus Tests
Middle Aged
Radiation Tolerance / genetics*
Radiation-Sensitizing Agents / toxicity*
Chemical
Reg. No./Substance:
0/Benzamides; 0/Radiation-Sensitizing Agents; 3544-24-9/3-aminobenzamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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