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Microneedle Biosensor: A Method for Direct Label-free Real Time Protein Detection.
MedLine Citation:
PMID:  23355762     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Here we present the development of an array of electrical micro-biosensors in a microfluidic channel, called microneedle biosensors. A microneedle biosensor is a real-time, label-free, direct electrical detection platform, which is capable of high sensitivity detection, measuring the change in ionic current and impedance modulation, due to the presence or reaction of biomolecules such as proteins and nucleic acids. In this study, we successfully fabricated and electrically characterized the sensors and demonstrated successful detection of target protein. In this study, we used biotinylated bovine serum albumin as the receptor and streptavidin as the target analyte.
Authors:
Rahim Esfandyarpour; Hesaam Esfandyarpour; Mehdi Javanmard; James S Harris; Ronald W Davis
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Sensors and actuators. B, Chemical     Volume:  177     ISSN:  0925-4005     ISO Abbreviation:  Sens Actuators B Chem     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101149755     Medline TA:  Sens Actuators B Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  848-855     Citation Subset:  -    
Affiliation:
Center for Integrated Systems, Department of Electrical Engineering, Stanford University ; Stanford Genome Technology Center; 855 California Ave., Palo Alto, CA 94304, USA.
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