| Micrometer-sized iron oxide particle labeling of mesenchymal stem cells for magnetic resonance imaging-based monitoring of cartilage tissue engineering. | |
| | |
MedLine Citation:
|
PMID: 20863643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To examine mesenchymal stem cell (MSC) labeling with micrometer-sized iron oxide particles (MPIOs) for magnetic resonance imaging (MRI)-based tracking and its application to monitoring articular cartilage regeneration. METHODS: Rabbit MSCs were labeled using commercial MPIOs. In vitro MRI was performed with gradient echo (GRE) and spin echo (SE) sequences at 3T and quantitatively characterized using line profile and region of interest analysis. Ex vivo MRI of hydrogel-encapsulated labeled MSCs implanted within a bovine knee was performed with spoiled GRE (SPGR) and T(1ρ) sequences. Fluorescence microscopy, labeling efficiency, and chondrogenesis of MPIO-labeled cells were also examined. RESULTS: MPIO labeling results in efficient contrast uptake and signal loss that can be visualized and quantitatively characterized via MRI. SPGR imaging of implanted cells results in ex vivo detection within native tissue, and T(1ρ) imaging is unaffected by the presence of labeled cells immediately following implantation. MPIO labeling does not affect quantitative glycosaminoglycan production during chondrogenesis, but iron aggregation hinders extracellular matrix visualization. This aggregation may result from excess unincorporated particles following labeling and is an issue that necessitates further investigation. CONCLUSION: This study demonstrates the promise of MPIO labeling for monitoring cartilage regeneration and highlights its potential in the development of cell-based tissue engineering strategies. |
| | |
Authors:
|
Karl J Saldanha; Ryan P Doan; Kristy M Ainslie; Tejal A Desai; Sharmila Majumdar |
Related Documents
:
|
16032673 - Magnetic resonance imaging and mathematical modeling of progressive formalin fixation o... 18645783 - A quantitative assessment of haemosiderosis in wild and captive birds using image analy... 10646123 - Analysis of ovariectomy and estrogen effects on body composition in rats by x-ray and m... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-21 |
Journal Detail:
|
Title: Magnetic resonance imaging Volume: 29 ISSN: 1873-5894 ISO Abbreviation: Magn Reson Imaging Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-14 Completed Date: 2011-04-12 Revised Date: 2012-01-04 |
Medline Journal Info:
|
Nlm Unique ID: 8214883 Medline TA: Magn Reson Imaging Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 40-9 Citation Subset: IM |
Copyright Information:
|
Published by Elsevier Inc. |
Affiliation:
|
MQIR, Department of Radiology, University of California, San Francisco, CA 94158, USA. kjs@berkeley.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cartilage, Articular / cytology*, growth & development* Cattle Cell Tracking / methods Cells, Cultured Female Ferric Compounds / diagnostic use* Fractures, Cartilage / pathology*, surgery* Magnetic Resonance Imaging / methods* Mesenchymal Stem Cell Transplantation / methods Mesenchymal Stem Cells / cytology* Microspheres Rabbits Regeneration / physiology Staining and Labeling / methods Tissue Engineering / methods Treatment Outcome |
| Grant Support | |
ID/Acronym/Agency:
|
R01 AG017762-10/AG/NIA NIH HHS; R01-AG17762/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Ferric Compounds; 1309-37-1/ferric oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Flow compensation for the fast spin echo triple-echo Dixon sequence.
Next Document: Comparison of competitive ligand-binding assay and bioassay formats for the measurement of neutraliz...