Document Detail


Micromanipulation of single cells from tissue imprints is an alternative to laser-assisted microdissection.
MedLine Citation:
PMID:  16008689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The characterization and analysis of single cells by molecular biological methods such as the polymerase chain reaction (PCR) is of increasing interest in biomedical research. Different techniques have been developed to obtain single cells from solid tissue. Currently, the most frequently used technique is laser-assisted microdissection (LAM). However, LAM of tissues cannot exclude contamination of the targeted cells by underlying cell fragments. Moreover, this technique can only be performed if a laser microscope is available. Thus, we developed a method to obtain single cells of fresh solid tissue by the simple technique of tissue imprints. After immunostaining of the imprints, single cells were transferred to a reaction tube using a 27-gauge needle guided by a mechanical micromanipulator. Consequently, we used these cells in a single cell PCR.
Authors:
Tilmann C Brauns; Manfred Goos
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of cutaneous pathology     Volume:  32     ISSN:  0303-6987     ISO Abbreviation:  J. Cutan. Pathol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-12     Completed Date:  2005-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425124     Medline TA:  J Cutan Pathol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  461-6     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Venerology and Allergology, University of Essen, Essen, Germany. tilmannbrauns@aol.com
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cytodiagnosis / instrumentation,  methods*
Female
Histocytological Preparation Techniques / methods*
Humans
Immunohistochemistry
Lasers*
Microdissection / methods*
Micromanipulation / methods*
Molecular Sequence Data
Mycosis Fungoides / genetics,  pathology
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, gamma-delta / genetics
Sequence Analysis, DNA
Skin / pathology*
Skin Neoplasms / genetics,  pathology
Chemical
Reg. No./Substance:
0/Receptors, Antigen, T-Cell, gamma-delta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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