| Micromachined electrochemical T-switches for cell sorting applications. | |
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MedLine Citation:
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PMID: 16234948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MEMS micro-T-switches actuated via electrochemical bubbles for cell sorting applications in a monolithic chip level are proposed and successfully demonstrated. The electrolysis-bubble actuator, which has the features of low operation temperature and high surface-tension force, is developed to actuate the micro-T-switch sorting structure in our device. The double T-structure design, the T-shape microchannel with the movable micro-T-switch structure located at the junction of the T-shape microchannel, with the electrolysis-bubble actuator makes an active-binary switch function available for cell sorting applications. The room temperature operation and the low voltage required for electrolysis actuation minimize the possibility of cell-damage that happens in the conventional high electric separation instruments, such as flow cytometry. The function of our micro-T-switch chip with a low required actuation voltage of 3.0 approximately 3.5 V is demonstrated by using human hepatoma cells in this paper. The pH-value measurements characterize the pH-value variation and distribution in the actuating chambers and the mainstream microchannels to trace the possible liver-cell injury due to the pH-value variation during electrolysis-actuation operation. The 84.1% cell viability in the sorted human hepatoma cells through our micro-T-switch sorter is observed via the fluorescence assay technique. Furthermore, 70.2% of total injected cells recover in culture after sorting and grow into colonies after micro-T-switch sorting operation. In this paper, we describe the design, microfabrication, and characterization of our micro-T-switch cell-sorting chip. We also report the cell-sorting demonstration and the cell viability results for the mammalian liver cells through our micro-T-switch cell-sorting chip. |
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Authors:
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Chen-Ta Ho; Ruei-Zeng Lin; Hwan-You Chang; Cheng-Hsien Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-21 |
Journal Detail:
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Title: Lab on a chip Volume: 5 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-12-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 1248-58 Citation Subset: IM |
Affiliation:
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Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 300, R.O.C. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Separation / instrumentation*, methods* Cell Survival Electrochemistry Hepatocytes / cytology Humans Hydrogen-Ion Concentration Microfluidic Analytical Techniques / instrumentation*, methods* Temperature |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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