| Micromachined Coulter counter for dynamic impedance study of time sensitive cells. | |
| | |
MedLine Citation:
|
PMID: 22526683 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This paper describes the design, modeling, fabrication and characterization MEMS Coulter counter that can detect and monitor the dynamic cell impedance changes in situ as a function of time after mixing isolated cell populations with different extracellular media within 0.3 s from the start of mixing. The novelty of this design is the use of multi-electrodes with vertical sidewalls to enable the measurements of time sensitive cells with significantly enhanced sensitivity as well as the integration of passive mixing, focusing of cells in line and impedance detection using the vertical electrodes on a single chip that is made mainly using multilayer of SU-8, which has not been reported before. The devices were tested with both fluidic and electrical functionality using yeast cells in cryoprotectant agent (diluted dimethyl sulfoxide), red blood cells, microbeads with different dimensions, and dyed fluids. The results demonstrate rapid changes of cell volume within the first 0.6 s after mixing followed by a stable and a fixed cell volume. The micromixer was initially simulated using COMSOL finite element tool. Image processing technique was used to quantitatively evaluate mixing efficiency by analyzing color intensities variation of captured images of 2 dyed fluids mixed in the channel at flow rates between 0.1-0.4 μl/min, the mixing efficiencies were between 87 %-95 %, respectively. |
| | |
Authors:
|
Yifan Wu; Xu Han; James D Benson; Mahmoud Almasri |
Related Documents
:
|
22938763 - Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelia... 23022133 - Uptake, efflux, and mass transfer coefficient of fluorescent pamam dendrimers into panc... 2160483 - Effects of electrical polarization on inner hair cell receptor potentials. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biomedical microdevices Volume: 14 ISSN: 1572-8781 ISO Abbreviation: Biomed Microdevices Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-08-08 Completed Date: 2012-12-13 Revised Date: 2013-04-23 |
Medline Journal Info:
|
Nlm Unique ID: 100887374 Medline TA: Biomed Microdevices Country: United States |
Other Details:
|
Languages: eng Pagination: 739-50 Citation Subset: IM |
Affiliation:
|
University of Missouri, Columbia, MO, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Membrane Permeability Cell Separation Cell Size* Cell Survival Dielectric Spectroscopy Electrodes Erythrocytes / cytology Extracellular Space / metabolism Hydrodynamics Microspheres Microtechnology / instrumentation* Saccharomyces cerevisiae / cytology Temperature Time Factors |
| Comments/Corrections | |
Erratum In:
|
Biomed Microdevices. 2013 Apr;15(2):381 Note: Han, Xu [added] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development of a microplate reader compatible microfluidic chip for ELISA.
Next Document: ??Tetrahydrocannabinol impairs visuo-spatial associative learning and spatial working memory in rhes...