| Microinjection technique: routine system for characterization of microcapillaries by bubble pressure measurement. | |
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MedLine Citation:
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PMID: 8299724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five tubing types with different outer and inner diameter dimensions were used to pull injection capillaries with different openings. The correlation between threshold bubble pressure and tip inner and outer diameter was established for each type of tubing. Statistical analysis revealed that the bubble pressure is an accurate measure for the tip inner diameter independent of the tubing used to pull the capillary. A graph directly relating the tip inner diameter to the threshold bubble pressure is presented. The tip outer diameter could not be related to the bubble pressure for tubings of different size. This diameter depends on the wall thickness of the tubing and the puller configuration. Data on the inner tip diameter were used to test the relationship between volume released from injection capillaries and their tip radius. It was found that the volume increases with the fourth power of the radius. Strategies for optimizing the injection capillary are discussed. |
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Authors:
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M Schnorf; I Potrykus; G Neuhaus |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 210 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-03-10 Completed Date: 1994-03-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 260-7 Citation Subset: IM |
Affiliation:
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Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Capillarity Microinjections / instrumentation, methods* Microscopy, Electron, Scanning Needles Pressure Regression Analysis |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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