Document Detail


Microinjection technique: routine system for characterization of microcapillaries by bubble pressure measurement.
MedLine Citation:
PMID:  8299724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five tubing types with different outer and inner diameter dimensions were used to pull injection capillaries with different openings. The correlation between threshold bubble pressure and tip inner and outer diameter was established for each type of tubing. Statistical analysis revealed that the bubble pressure is an accurate measure for the tip inner diameter independent of the tubing used to pull the capillary. A graph directly relating the tip inner diameter to the threshold bubble pressure is presented. The tip outer diameter could not be related to the bubble pressure for tubings of different size. This diameter depends on the wall thickness of the tubing and the puller configuration. Data on the inner tip diameter were used to test the relationship between volume released from injection capillaries and their tip radius. It was found that the volume increases with the fourth power of the radius. Strategies for optimizing the injection capillary are discussed.
Authors:
M Schnorf; I Potrykus; G Neuhaus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  210     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-10     Completed Date:  1994-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  260-7     Citation Subset:  IM    
Affiliation:
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich.
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MeSH Terms
Descriptor/Qualifier:
Capillarity
Microinjections / instrumentation,  methods*
Microscopy, Electron, Scanning
Needles
Pressure
Regression Analysis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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