Document Detail

Microinjection technique: routine system for characterization of microcapillaries by bubble pressure measurement.
MedLine Citation:
PMID:  8299724     Owner:  NLM     Status:  MEDLINE    
Five tubing types with different outer and inner diameter dimensions were used to pull injection capillaries with different openings. The correlation between threshold bubble pressure and tip inner and outer diameter was established for each type of tubing. Statistical analysis revealed that the bubble pressure is an accurate measure for the tip inner diameter independent of the tubing used to pull the capillary. A graph directly relating the tip inner diameter to the threshold bubble pressure is presented. The tip outer diameter could not be related to the bubble pressure for tubings of different size. This diameter depends on the wall thickness of the tubing and the puller configuration. Data on the inner tip diameter were used to test the relationship between volume released from injection capillaries and their tip radius. It was found that the volume increases with the fourth power of the radius. Strategies for optimizing the injection capillary are discussed.
M Schnorf; I Potrykus; G Neuhaus
Related Documents :
17963414 - The drop size in membrane emulsification determined from the balance of capillary and h...
16529854 - Effect of externally applied electrostatic fields on the surface topography of ceramide...
21305824 - Sex differences in blood pressure levels and its association with obesity indices: who ...
9255654 - Drawover vaporizers for sedation in intensive care.
24779804 - Nice issues guidance on pressure ulcer prevention and management.
20937994 - Frequent loss of nyctohemeral rhythm of intraocular pressure restored by ncpap treatmen...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  210     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-10     Completed Date:  1994-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  260-7     Citation Subset:  IM    
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Microinjections / instrumentation,  methods*
Microscopy, Electron, Scanning
Regression Analysis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Metalloproteinase-dependent neurite outgrowth within a synthetic extracellular matrix is induced by ...
Next Document:  Expression of three alternative acetylcholinesterase messenger RNAs in human tumor cell lines of dif...