| Microinjection of follicle-enclosed mouse oocytes. | |
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MedLine Citation:
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PMID: 19085139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mammalian oocyte develops within a complex of somatic cells known as a follicle, within which signals from the somatic cells regulate the oocyte, and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative microinjection of follicle-enclosed mouse oocytes, thus allowing the introduction of signaling molecules as well as optical probes into the oocyte within its physiological environment. |
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Authors:
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Laurinda A Jaffe; Rachael P Norris; Marina Freudzon; William J Ratzan; Lisa M Mehlmann |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 518 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-02-11 Revised Date: 2013-06-04 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 157-73 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Dioxide Cells, Cultured Dissection Female Humidity Mice Microinjections / methods* Oocytes / cytology, metabolism* Ovarian Follicle / cytology*, metabolism Temperature |
| Grant Support | |
ID/Acronym/Agency:
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R01 HD014939/HD/NICHD NIH HHS; R01 HD014939-26/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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