Document Detail


Microinjection of follicle-enclosed mouse oocytes.
MedLine Citation:
PMID:  19085139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mammalian oocyte develops within a complex of somatic cells known as a follicle, within which signals from the somatic cells regulate the oocyte, and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative microinjection of follicle-enclosed mouse oocytes, thus allowing the introduction of signaling molecules as well as optical probes into the oocyte within its physiological environment.
Authors:
Laurinda A Jaffe; Rachael P Norris; Marina Freudzon; William J Ratzan; Lisa M Mehlmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  518     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-11     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-73     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide
Cells, Cultured
Dissection
Female
Humidity
Mice
Microinjections / methods*
Oocytes / cytology,  metabolism*
Ovarian Follicle / cytology*,  metabolism
Temperature
Grant Support
ID/Acronym/Agency:
R01 HD014939/HD/NICHD NIH HHS; R01 HD014939-26/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide
Comments/Corrections

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