Document Detail

Microinjection as a tool of mechanical delivery.
MedLine Citation:
PMID:  18725294     Owner:  NLM     Status:  MEDLINE    
Microinjection to single cells has been widely used in the studies of transduction-challenged cells, transgenic animal production, and in vitro fertilization to mechanically transfer DNAs, RNA interferences, sperms, proteins, peptides, and drugs. The advantages of microinjection include the precision of delivery dosage and timing, high efficiency of transduction as well as low cytotoxicity. However, manual microinjection is labor intensive and time consuming, which limits the application of this technique to large number of cells in a sample. New cell culture matrix ensuring all cells grow in a desired position and orientation is needed for application of high throughput automatic injection systems, which will significantly increase injection speed, cell survival, and success rates.
Yan Zhang; Long-Chuan Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2008-09-03
Journal Detail:
Title:  Current opinion in biotechnology     Volume:  19     ISSN:  0958-1669     ISO Abbreviation:  Curr. Opin. Biotechnol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100492     Medline TA:  Curr Opin Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  506-10     Citation Subset:  IM    
Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
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MeSH Terms
Drug Delivery Systems / instrumentation*,  methods*,  trends
Gene Transfer Techniques / instrumentation*,  trends
Mechanical Phenomena
Microinjections / instrumentation*,  methods*,  trends

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