Document Detail

Microinjection of antibodies to the calpactin I light chain in MDBK cells causes precipition of the cytoskeletal calpactin I complex without affecting the distribution of related proteins.
MedLine Citation:
PMID:  2144634     Owner:  NLM     Status:  MEDLINE    
The calpactin I complex is composed of two heavy chain (39K) and two light chain (11K) subunits. The heavy chain is a member of a protein family that includes lipocortins, endonexin and chromobindins while the light chain is a member of the S100 family (7 distinct members are known). We have found that the kidney epithelial cell line MDBK expresses four members of the heavy chain family and two members of the light chain protein family. Antibodies to the light chain of calpactin I were found to cause the precipitation of injected antibody together with the associated heavy chain without apparent effect on the distribution of related proteins. This suggests a differential targeting of various members of the calpactin heavy and light chain families even within the same cell.
J R Glenney
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Progress in clinical and biological research     Volume:  349     ISSN:  0361-7742     ISO Abbreviation:  Prog. Clin. Biol. Res.     Publication Date:  1990  
Date Detail:
Created Date:  1990-10-18     Completed Date:  1990-10-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7605701     Medline TA:  Prog Clin Biol Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  135-46     Citation Subset:  IM    
Markey Cancer Center, University of Kentucky, College of Medicine, Lexington 40536.
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MeSH Terms
Antibodies, Monoclonal / administration & dosage
Calcium-Binding Proteins / immunology,  metabolism*
Cell Line
Chemical Precipitation
Cytoskeleton / metabolism*
Fluorescent Antibody Technique
Protein Conformation
Reg. No./Substance:
0/Annexins; 0/Antibodies, Monoclonal; 0/Calcium-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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