Document Detail


Microinjection of anti-alpha-tubulin antibody (DM1A) inhibits progesterone-induced meiotic maturation and deranges the microtubule array in follicle-enclosed oocytes of the frog, Rana pipiens.
MedLine Citation:
PMID:  9223249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microinjection of anti-alpha-tubulin (Dm1A) inhibited progesterone-induced meiotic maturation in large follicle-enclosed oocytes of the frog, Rana pipiens. DM1A (46 nl; 10 mg/ml) injection significantly increased the ED50 value for progesterone as determined by germinal vesicle dissolution (GVD) bioassay. By contrast, low doses of microinjected DM1A (46 nl; 2.5 mg/ml), anti-actin (clone KJ43A), anti-cytokeratin (C-11), anti-intermediate filament antibody (IFA), generic IgG (46 nl; 20 mg/ml) or sodium azide (46 nl; 1 mg/ml), an antibody preservative, were without inhibitory effect in this bioassay. Microinjected, affinity-purified DM1A (46 nl; 7.5 mg/ml) was also inhibitory, but preabsorption with pure tubulin prior to injection significantly reduced the inhibitory effect. DM1A injection had no effect on centrifugation-induced germinal vesicle migration (GVM). Previous work indicated that drugs (e.g. demecolcine and nocodazole), which destabilise microtubules, enhance both centrifugation-induced GVM and progesterone-induced GVD in Rana oocytes. Taking these results together, it is suggested that DM1A injection may have differential effects on microtubules in this cell. Thus, while the majority of microtubules were apparently depolymerised by DM1A (46 nl; 10 mg/ml) injection, a small subpopulation appeared to be stabilised as bundles. Confocal immunofluorescence microscopy of follicle-enclosed oocytes after DM1A injection revealed a major loss of microtubules throughout the cell; however, apparent sparse bundles of microtubules arranged in an approximately 600 microns shell were associated with the injectate region 24 h post-injection. By contrast, control follicle-enclosed oocytes topically labelled with DM1A post-fixation had extensive microtubule arrays similar to those previously reported in Xenopus oocytes. Intracellular recording after DM1A injection and progesterone treatment yielded an intermediate membrane potential (Vm = -31.8 mV) compared with control (immature) DM1A-injected cells (Vm = -44.7 mV) or potassium balanced salt solution (KBS)-injected cells matured with progesterone (Vm = -13.9 mV). These results suggest that DM1A injection does not completely inhibit electrophysiological changes initiated by progesterone. Working hypotheses are proposed that suggest a role for microtubules in the action of progesterone which normally lifts the prophase I block in the Rana follicle-enclosed oocyte.
Authors:
C A Lessman; T Wang; D L Gard; C W Woods
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  5     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-08-22     Completed Date:  1997-08-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  83-95     Citation Subset:  IM    
Affiliation:
Department of Microbiology & Molecular Cell Sciences, University of Memphis, Tennessee 38152-6041, USA. lessmanca@msuvxl.memphis.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology,  pharmacology*
Female
Meiosis / drug effects*
Membrane Potentials
Microinjections
Microtubules / drug effects*
Oocytes / drug effects*,  physiology,  ultrastructure
Ovarian Follicle / cytology,  drug effects
Progesterone / pharmacology*
Rana pipiens
Tubulin / immunology*
Grant Support
ID/Acronym/Agency:
HD-26901/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Tubulin; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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