| Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment. | |
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MedLine Citation:
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PMID: 15603808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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For several years, microgrooved substrates have been evaluated as a means to orient cells in engineered tissues. Recently, we fabricated thin (0.1-5.3 microm) planar and tubular collagen membranes (CMs) from air-dried hydrogels of native, fibrillar type I collagen (Vernon et al., Biomaterials 2004;26:1109-17). The CMs were strong, stable, and permeable and, hence, of potential use as scaffolds for tissue engineering. In the present study, planar CMs supported a robust attachment, spreading, and proliferation of human dermal fibroblasts (HDFs) and human umbilical artery smooth muscle cells (HUASMCs). Collagen hydrogels were air-dried onto microgrooved templates and subsequently removed in the form of grooved CMs with the potential to align cells. The grooved CMs were highly effective at inducing HDFs and HUASMCs to elongate and align, as revealed by scanning electron microscopy and by assays of f-actin and nuclear orientation. Alignment of cells was maintained at high cell densities. CMs with grooves of substantially different widths and depths were similarly effective in causing cell alignment; however, cells aligned poorly on CMs that had grooves less than 1 microm in depth. Grooved CMs with the capability to align cells might be of considerable use in the fabrication of tissue substitutes. |
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Authors:
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Robert B Vernon; Michel D Gooden; Stephanie L Lara; Thomas N Wight |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biomaterials Volume: 26 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2004-12-17 Completed Date: 2005-06-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 3131-40 Citation Subset: IM |
Affiliation:
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Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101-2795, USA. rvernon@hopeheart.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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chemistry Biocompatible Materials / chemistry* Cell Adhesion Cell Nucleus / metabolism Cell Proliferation Cells, Cultured Collagen Type I / chemistry Endothelium, Vascular / cytology Extracellular Matrix / metabolism Fibrillar Collagens / chemistry* Fibroblasts / cytology, metabolism Humans Hydrogel / chemistry Hydrogels / chemistry Microscopy, Electron, Scanning Myocytes, Smooth Muscle / cytology Surface Properties Time Factors Tissue Engineering Umbilical Veins / cytology |
| Grant Support | |
ID/Acronym/Agency:
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R24HL64387/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Biocompatible Materials; 0/Collagen Type I; 0/Fibrillar Collagens; 0/Hydrogels; 25852-47-5/Hydrogel |
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