Document Detail

Microgrooved fibrillar collagen membranes as scaffolds for cell support and alignment.
MedLine Citation:
PMID:  15603808     Owner:  NLM     Status:  MEDLINE    
For several years, microgrooved substrates have been evaluated as a means to orient cells in engineered tissues. Recently, we fabricated thin (0.1-5.3 microm) planar and tubular collagen membranes (CMs) from air-dried hydrogels of native, fibrillar type I collagen (Vernon et al., Biomaterials 2004;26:1109-17). The CMs were strong, stable, and permeable and, hence, of potential use as scaffolds for tissue engineering. In the present study, planar CMs supported a robust attachment, spreading, and proliferation of human dermal fibroblasts (HDFs) and human umbilical artery smooth muscle cells (HUASMCs). Collagen hydrogels were air-dried onto microgrooved templates and subsequently removed in the form of grooved CMs with the potential to align cells. The grooved CMs were highly effective at inducing HDFs and HUASMCs to elongate and align, as revealed by scanning electron microscopy and by assays of f-actin and nuclear orientation. Alignment of cells was maintained at high cell densities. CMs with grooves of substantially different widths and depths were similarly effective in causing cell alignment; however, cells aligned poorly on CMs that had grooves less than 1 microm in depth. Grooved CMs with the capability to align cells might be of considerable use in the fabrication of tissue substitutes.
Robert B Vernon; Michel D Gooden; Stephanie L Lara; Thomas N Wight
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomaterials     Volume:  26     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2004-12-17     Completed Date:  2005-06-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3131-40     Citation Subset:  IM    
Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101-2795, USA.
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MeSH Terms
Actins / chemistry
Biocompatible Materials / chemistry*
Cell Adhesion
Cell Nucleus / metabolism
Cell Proliferation
Cells, Cultured
Collagen Type I / chemistry
Endothelium, Vascular / cytology
Extracellular Matrix / metabolism
Fibrillar Collagens / chemistry*
Fibroblasts / cytology,  metabolism
Hydrogel / chemistry
Hydrogels / chemistry
Microscopy, Electron, Scanning
Myocytes, Smooth Muscle / cytology
Surface Properties
Time Factors
Tissue Engineering
Umbilical Veins / cytology
Grant Support
Reg. No./Substance:
0/Actins; 0/Biocompatible Materials; 0/Collagen Type I; 0/Fibrillar Collagens; 0/Hydrogels; 25852-47-5/Hydrogel

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