Document Detail


Microgravity effects on leaf morphology, cell structure, carbon metabolism and mRNA expression of dwarf wheat.
MedLine Citation:
PMID:  16708225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of higher plants as the basis for a biological life support system that regenerates the atmosphere, purifies water, and produces food has been proposed for long duration space missions. The objective of these experiments was to determine what effects microgravity (microg) had on chloroplast development, carbohydrate metabolism and gene expression in developing leaves of Triticum aestivum L. cv. USU Apogee. Gravity naive wheat plants were sampled from a series of seven 21-day experiments conducted during Increment IV of the International Space Station. These samples were fixed in either 3% glutaraldehyde or RNAlater or frozen at -25 degrees C for subsequent analysis. In addition, leaf samples were collected from 24- and 14-day-old plants during the mission that were returned to Earth for analysis. Plants grown under identical light, temperature, relative humidity, photoperiod, CO(2), and planting density were used as ground controls. At the morphological level, there was little difference in the development of cells of wheat under microg conditions. Leaves developed in mug have thinner cross-sectional area than the 1g grown plants. Ultrastructurally, the chloroplasts of microg grown plants were more ovoid than those developed at 1g, and the thylakoid membranes had a trend to greater packing density. No differences were observed in the starch, soluble sugar, or lignin content of the leaves grown in microg or 1g conditions. Furthermore, no differences in gene expression were detected leaf samples collected at microg from 24-day-old leaves, suggesting that the spaceflight environment had minimal impact on wheat metabolism.
Authors:
G W Stutte; O Monje; R D Hatfield; A-L Paul; R J Ferl; C G Simone
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-05-10
Journal Detail:
Title:  Planta     Volume:  224     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-21     Completed Date:  2007-03-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1038-49     Citation Subset:  IM    
Affiliation:
Space Life Sciences Laboratory, Dynamac Corporation, Mail Code Dyn-3, Kennedy Space Center, Kennedy, FL 32899, USA. stuttgw@kscems.ksc.nasa.gov
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Metabolism*
Cell Wall / metabolism
Chloroplasts / physiology
Gene Expression*
Plant Leaves / cytology,  growth & development*
RNA, Messenger
Space Flight
Starch / metabolism
Triticum / cytology,  growth & development*,  metabolism
Weightlessness*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9005-25-8/Starch

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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