Document Detail

Microglial cell death induced by glycated bovine serum albumin: nitric oxide involvement.
MedLine Citation:
PMID:  18463114     Owner:  NLM     Status:  MEDLINE    
Nonenzymatic glycation results in the formation of advanced glycation end products (AGEs) through a nonenzymatic multistep reaction of reducing sugars with proteins. AGEs have been suspected to be involved in the pathogenesis of several chronic clinical neurodegenerative complications including Alzheimer's disease, which is characterized with the activation of microglial cells in neuritic plaques. To find out the consequence of this activation on microglial cells, we treated the cultured microglial cells with different glycation levels of Bovine Serum Albumin (BSA) which were prepared in vitro. Extent of glycation of protein has been characterized during 16 weeks of incubation with glucose. Treatment of microglial cells with various levels of glycated albumin induced nitric oxide (NO) production and consequently cell death. We also tried to find out the mode of death in AGE-activated microglial cells. Altogether, our results suggest that AGE treatment causes microglia to undergo NO-mediated apoptotic and necrotic cell death in short term and long term, respectively. NO production is a consequence of iNOS expression in a JNK dependent RAGE signalling after activation of RAGE by AGE-BSA.
Mohammad R Khazaei; Mehran Habibi-Rezaei; Fereshteh Karimzadeh; Ali Akbar Moosavi-Movahedi; Abdo Alfattah Sarrafnejhad; Farzaneh Sabouni; Mostafa Bakhti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-07
Journal Detail:
Title:  Journal of biochemistry     Volume:  144     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-04     Completed Date:  2008-10-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  197-206     Citation Subset:  IM    
School of Biology, College of Science, Department of Health, Medical Sciences, University of Tehran, Tehran, Iran.
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MeSH Terms
Annexin A5 / analysis
Cell Survival / drug effects
Cells, Cultured
Glycosylation End Products, Advanced / chemistry,  pharmacology*
JNK Mitogen-Activated Protein Kinases / metabolism
MAP Kinase Signaling System
Microglia / cytology,  enzymology*,  metabolism
Nitric Oxide / biosynthesis*
Nitric Oxide Synthase Type II / biosynthesis
Rats, Wistar
Receptors, Immunologic / metabolism
Serum Albumin / chemistry,  pharmacology*
Reg. No./Substance:
0/Annexin A5; 0/Glycosylation End Products, Advanced; 0/Receptors, Immunologic; 0/Serum Albumin; 0/advanced glycosylation end-product receptor; 0/glycosylated serum albumin; 10102-43-9/Nitric Oxide; EC Oxide Synthase Type II; EC Mitogen-Activated Protein Kinases

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