Document Detail


Microglial and astrocytic cell responses in the rat hippocampus after an intracerebroventricular kainic acid injection.
MedLine Citation:
PMID:  7687961     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After central nervous system injury activated microglial cells and reactive astrocytes secrete neurotrophic factors which may provide an environment conducive to axonal sprouting. The present study has used a unilateral intracerebroventricular (ICV) injection of kainic acid (KA) to produce a lesion of the CA3 pyramidal neurons in the rat hippocampus. The time course of the microglial and astrocytic response was studied throughout a 3-month period using an antibody to proliferating cell nuclear antigen to identify proliferating cells as well as OX-42 and GFAP antibodies to identify the activated microglia and reactive astrocytes, respectively. There was no proliferation of reactive astrocytes whereas activated microglial cells continued to proliferate throughout the duration of the study. During the first month there were some activated microglial cells in the CA1 field and in the fascia dentata but this was short-lived in comparison to the persistence of activated microglia and reactive astrocytes in the CA3 field which were still present 3 months after the initial injection. The discussion attempts to correlate this ipsilateral microglial and astrocytic response with the bilateral mossy fiber axonal sprouting, which occurs in the dentate gyrus after a unilateral ICV injection of KA. The discussion concludes that the two events, the microglial and astrocytic response and the mossy fiber sprouting, are not directly related since the contralateral sprouting occurs in the absence of any astrocytic or microglial response.
Authors:
J Mitchell; L E Sundstrom; H V Wheal
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental neurology     Volume:  121     ISSN:  0014-4886     ISO Abbreviation:  Exp. Neurol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-08-31     Completed Date:  1993-08-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  224-30     Citation Subset:  IM    
Affiliation:
Department of Human Morphology, University of Southampton, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / cytology*,  drug effects
Cell Division
Hippocampus / cytology*,  drug effects
Injections, Intraventricular
Kainic Acid / administration & dosage,  pharmacology*
Male
Nerve Fibers / drug effects
Neuroglia / cytology*,  drug effects
Rats
Rats, Wistar
Staining and Labeling
Chemical
Reg. No./Substance:
487-79-6/Kainic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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