Document Detail


Microglial activation and expression of immune-related genes in a rat ex vivo nervous system model after infection with Listeria monocytogenes.
MedLine Citation:
PMID:  23322603     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A wide variety of microorganisms has previously been identified as causes of brain infection. Among them, Listeria monocytogenes has a particular tropism for the central nervous system. To gain knowledge about the immune response elicited by L. monocytogenes in the brain, we used a rat ex vivo organotypic nervous system culture as a model for Listeria infection. Scanning electron microscopy (SEM) revealed that activated microglial cells showing a typical amoeboid morphology are quickly recruited to the surface of the explants after the infection. After bacterial engulfment, these cells appear to act as Trojan horses, releasing the engulfed bacteria inside the brain tissue. We describe cycles of microglial phagocytosis, necrotic cell death and the subsequent removal of cell debris for the first time. Furthermore, we used this ex vivo model to assess the expression profiles of immune relevant genes up to 24 h postinfection by means of q-PCR-arrays, finding that a number of inflammation-promoting genes are upregulated. Shortly after infection by L. monocytogenes, upregulated genes were those that encoded molecules involved in Th1 responses, being the Ccl2 chemokine and members of the interleukin1-β family the most abundant immunomodulatory signals expressed. After 5 h of infection, L. monocytogenes caused a substantial increase in the expression of TLR1 and TLR2 genes, as well as in several downstream genes of the TLR signaling pathways. © 2013 Wiley Periodicals, Inc.
Authors:
Sara Remuzgo-Martínez; Lilian Pilares-Ortega; Jose M Icardo; Elsa Maria Valdizán; Verónica Inés Vargas; Angel Pazos; Jose Ramos-Vivas
Related Documents :
8605293 - Regulation, expression and function of a new basic chitinase gene in rice (oryza sativa...
23757413 - Lentiviruses allow widespread and conditional manipulation of gene expression in the de...
6098683 - Dna rearrangements of the actin gene cluster in caenorhabditis elegans accompany revers...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Glia     Volume:  -     ISSN:  1098-1136     ISO Abbreviation:  Glia     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla-IFIMAV, Santander, Cantabria, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Deciphering of ADP-induced, phosphotyrosine-dependent signaling networks in human platelets by SH2 p...
Next Document:  Detection and distribution of putative virulence associated genes in Aeromonas species from freshwat...