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Microglia-like or microglia: results of the weak silver carbonate staining method of del Rio-Hortega.
MedLine Citation:
PMID:  22404562     Owner:  NLM     Status:  Publisher    
We investigated the character and origin of the newly described "microglia-like" cell in the mammalian organ of Corti after aminoglycoside intoxication. We replicated the neomycin-induced ototoxicity model in cochleae of neonatal Sprague-Dawley rats and used their brains as microglia positive controls. The weak silver carbonate staining method of del Rio-Hortega was used to identify the microglia-like cell. The microglia-like cell was confirmed by transmission electron microscope (TEM). Microglia in the brain were stained while rat microglia-like cells in the cochlea were unstained by the weak silver carbonate staining method of del Rio-Hortega. Because the microglia-like cell was unstained by the method of del Rio-Hortega, it is unlikely that the newly found cell is related to microglia.
Yucheng Wang; W Wei
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-12
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  -     ISSN:  1473-7760     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital of Fudan University.
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