Document Detail


Microgenomics: Identification of new expression profiles via small and single-cell sample analyses.
MedLine Citation:
PMID:  15170605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Since the sequencing of the human genome has been finished, microgenomics has been booming, employing highly sophisticated, high-throughput platforms. But these mainly chip-based methods can only generate biologically relevant data if the samples investigated consist of homogeneous cell populations, in which no unwanted cells of different specificity and/or developmental stage obscure the results. METHODS: Different sampling methods have been routinely applied to overcome the problem presented by heterogeneous samples, e.g., global surveys, cell cultures, and microdissection. Various methods of laser-assisted microdissection, employing either positive or negative selection of tissue areas or even single cells, are available. RESULTS: These laser-assisted microdissection methods allow for fast and precise procurement of extremely small samples. Through subsequent application of recently developed methods of linear mRNA amplification in a pool of isolated total RNA, it has now become possible to perform complex high-throughput RNA expression profiling by microdissecting and processing even single-cell samples. CONCLUSIONS: Studies using the tools and methods of microgenomics have shed light on how those new approaches will eventually aid in the development of a new generation of diagnostics, e.g., leading to new patient-specific drugs tailored to the requirements assessed by assaying only a few biopsy cells.
Authors:
Theresa B Taylor; Prashant R Nambiar; Rajiv Raja; Evelyn Cheung; Daniel W Rosenberg; Birgit Anderegg
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  59     ISSN:  1552-4922     ISO Abbreviation:  Cytometry A     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-01     Completed Date:  2004-12-13     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  254-61     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Wiley-Liss, Inc.
Affiliation:
Arcturus Bioscience, Inc., Mountain View, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / methods*
Cytogenetic Analysis / methods*
Gene Expression Profiling*
Genomics / methods*
Humans
Lasers
Microdissection / methods
RNA, Messenger / analysis*,  genetics
Chemical
Reg. No./Substance:
0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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