Document Detail


Microfluidics for cryopreservation.
MedLine Citation:
PMID:  19532962     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Minimizing cell damage throughout the cryopreservation process is critical to enhance the overall outcome. Osmotic shock sustained during the loading and unloading of cryoprotectants (CPAs) is a major source of cell damage during the cryopreservation process. We introduce a microfluidic approach to minimize osmotic shock to cells during cryopreservation. This approach allows us to control the loading and unloading of CPAs in microfluidic channels using diffusion and laminar flow. We provide a theoretical explanation of how the microfluidic approach minimizes osmotic shock in comparison to conventional cryopreservation protocols via cell membrane transport modeling. Finally, we show that biological experiments are consistent with the proposed mathematical model. The results indicate that our novel microfluidic-based approach improves post-thaw cell survivability by up to 25% on average over conventional cryopreservation protocols. The method developed in this study provides a platform to cryopreserve cells with higher viability, functionality, and minimal inter-technician variability. This method introduces microfluidic technologies to the field of biopreservation, opening the door to future advancements at the interface of these fields.
Authors:
Young S Song; Sangjun Moon; Leon Hulli; Syed K Hasan; Emre Kayaalp; Utkan Demirci
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-31
Journal Detail:
Title:  Lab on a chip     Volume:  9     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-17     Completed Date:  2009-08-11     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1874-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / metabolism
Cell Line, Tumor
Cell Membrane Permeability*
Cell Survival
Cryopreservation / instrumentation*,  methods*
Cryoprotective Agents / pharmacokinetics*
Equipment Design
Humans
Liver / cytology
Liver Neoplasms / metabolism
Microfluidic Analytical Techniques / instrumentation*,  methods
Models, Biological
Osmotic Pressure
Rheology
Grant Support
ID/Acronym/Agency:
R21 EB007707/EB/NIBIB NIH HHS; R21 EB007707/EB/NIBIB NIH HHS; R21 EB007707-01A1/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Cryoprotective Agents
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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