Document Detail


Microfluidic systems to examine intercellular coupling of pairs of cardiac myocytes.
MedLine Citation:
PMID:  17538715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper we describe a microfluidic environment that enables us to explore cell-to-cell signalling between longitudinally linked primary heart cells. We have chosen to use pairs (or doublets) of cardiac myocyte as a model system, not only because of the importance of cell-cell signalling in the study of heart disease but also because the single cardiomyocytes are both mechanically and electrically active and their synchronous activation due to the intercellular coupling within the doublet can be readily monitored on optical and electrical recordings. Such doublets have specialised intercellular contact structures in the form of the intercalated discs, comprising the adhesive junction (fascia adherens and macula adherens or desmosome) and the connecting junction (known as gap junction). The latter structure enables adjacent heart cells to share ions, second messengers and small metabolites (<1 kDa) between them and thus provides the structural basis for the synchronous (syncytical) behaviour of connected cardiomyocytes. Using the unique environment provided by the microfluidic system, described in this paper, we explore the local ionic conditions that enable the propagation of Ca(2+) waves between two heart cells. We observe that the ability of intracellular Ca(2+) waves to traverse the intercalated discs is dependent on the relative concentrations of diastolic Ca(2+) in the two adjacent cells. These experiments rely upon our ability to independently control both the electrical stimulation of each of the cells (using integrated microelectrodes) and to rapidly change (or switch) the local concentrations of ions and drugs in the extracellular buffer within the microfluidic channel (using a nanopipetting system). Using this platform, it is also possible to make simultaneous optical recordings (including fluorescence and cell contraction) to explore the effect of drugs on one or both cells, within the doublet.
Authors:
Norbert Klauke; Godfrey Smith; Jonathan M Cooper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-17
Journal Detail:
Title:  Lab on a chip     Volume:  7     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-31     Completed Date:  2007-08-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  731-9     Citation Subset:  IM    
Affiliation:
Department of Electronics, University of Glasgow, Glasgow, UK G12 8LT.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Channels / physiology*
Cell Adhesion Molecules / chemistry,  physiology
Electric Stimulation
Electrophysiology
Intercellular Junctions / physiology*,  ultrastructure
Microfluidics / methods*
Models, Biological
Myocytes, Cardiac / cytology,  physiology*,  ultrastructure
Optics and Photonics
Rabbits
Video Recording
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/Cell Adhesion Molecules

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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