Document Detail

Microfluidic sorting of microtissues.
MedLine Citation:
PMID:  22505992     Owner:  NLM     Status:  In-Data-Review    
Increasingly, invitro culture of adherent cell types utilizes three-dimensional (3D) scaffolds or aggregate culture strategies to mimic tissue-like, microenvironmental conditions. In parallel, new flow cytometry-based technologies are emerging to accurately analyze the composition and function of these microtissues (i.e., large particles) in a non-invasive and high-throughput way. Lacking, however, is an accessible platform that can be used to effectively sort or purify large particles based on analysis parameters. Here we describe a microfluidic-based, electromechanical approach to sort large particles. Specifically, sheath-less asymmetric curving channels were employed to separate and hydrodynamically focus particles to be analyzed and subsequently sorted. This design was developed and characterized based on wall shear stress, tortuosity of the flow path, vorticity of the fluid in the channel, sorting efficiency and enrichment ratio. The large particle sorting device was capable of purifying fluorescently labelled embryoid bodies (EBs) from unlabelled EBs with an efficiency of 87.3% ± 13.5%, and enrichment ratio of 12.2 ± 8.4 (n = 8), while preserving cell viability, differentiation potential, and long-term function.
D G Buschke; P Resto; N Schumacher; B Cox; A Tallavajhula; A Vivekanandan; K W Eliceiri; J C Williams; B M Ogle
Publication Detail:
Type:  Journal Article     Date:  2012-03-07
Journal Detail:
Title:  Biomicrofluidics     Volume:  6     ISSN:  1932-1058     ISO Abbreviation:  Biomicrofluidics     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-04-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101293825     Medline TA:  Biomicrofluidics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14116-1411611     Citation Subset:  -    
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