| Microfluidic single cell analysis: from promise to practice. | |
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MedLine Citation:
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PMID: 22525493 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Methods for single-cell analysis are critical to revealing cell-to-cell variability in biological systems, especially in cases where relevant minority cell populations can be obscured by population-averaged measurements. However, to date single cell studies have been limited by the cost and throughput required to examine large numbers of cells and the difficulties associated with analyzing small amounts of starting material. Microfluidic approaches are well suited to resolving these issues by providing increased senstitivity, economy of scale, and automation. After many years of development microfluidic systems are now finding traction in a variety of single-cell analytics including gene expression measurements, protein analysis, signaling response, and growth dynamics. With newly developed tools now being applied in fields ranging from human haplotyping and drug discovery to stem cell and cancer research, the long-heralded promise of microfluidic single cell analysis is now finally being realized. |
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Authors:
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Véronique Lecault; Adam K White; Anupam Singhal; Carl L Hansen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-21 |
Journal Detail:
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Title: Current opinion in chemical biology Volume: - ISSN: 1879-0402 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9811312 Medline TA: Curr Opin Chem Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Centre for High-Throughput Biology, University of British Columbia, 2185 East Mall, Vancouver, British Columbia, V6T 1Z4, Canada; Michael Smith Laboratories, University of British Columbia, 2125 East Mall, Vancouver, British Columbia, V6T 1Z4, Canada; Department of Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, British Columbia, V6T 1Z3, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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