Document Detail


Microfluidic sample preparation for diagnostic cytopathology.
MedLine Citation:
PMID:  23380972     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cellular components of body fluids are routinely analyzed to identify disease and treatment approaches. While significant focus has been placed on developing cell analysis technologies, tools to automate the preparation of cellular specimens have been more limited, especially for body fluids beyond blood. Preparation steps include separating, concentrating, and exposing cells to reagents. Sample preparation continues to be routinely performed off-chip by technicians, preventing cell-based point-of-care diagnostics, increasing the cost of tests, and reducing the consistency of the final analysis following multiple manually-performed steps. Here, we review the assortment of biofluids for which suspended cells are analyzed, along with their characteristics and diagnostic value. We present an overview of the conventional sample preparation processes for cytological diagnosis. We finally discuss the challenges and opportunities in developing microfluidic devices for the purpose of automating or miniaturizing these processes, with particular emphases on preparing large or small volume samples, working with samples of high cellularity, automating multi-step processes, and obtaining high purity subpopulations of cells. We hope to convey the importance of and help identify new research directions addressing the vast biological and clinical applications in preparing and analyzing the array of available biological fluids. Successfully addressing the challenges described in this review can lead to inexpensive systems to improve diagnostic accuracy while simultaneously reducing overall systemic healthcare costs.
Authors:
Albert J Mach; Oladunni B Adeyiga; Dino Di Carlo
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-20     Completed Date:  2013-08-27     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1011-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / cytology
Blood Cells / cytology
Body Fluids / cytology*
Bone Marrow Cells / cytology
Bronchoalveolar Lavage Fluid / cytology
Humans
Microfluidic Analytical Techniques*
Point-of-Care Systems
Specimen Handling
Synovial Fluid / cytology
Urine / cytology
Grant Support
ID/Acronym/Agency:
T32 AI089398/AI/NIAID NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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