Document Detail


Microfluidic flow-flash: method for investigating protein dynamics.
MedLine Citation:
PMID:  17194129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design utilizes three-dimensional sheath flow to reduce sample dispersion and minimize sample consumption. The ability to alter the flow rates in the microfluidic flow cells allows a variety of time scales to be studied with submillisecond time resolution. The imaging detection system can be coupled with several spectroscopic probes including fluorescence and UV/visible absorbance spectroscopy. Here, we utilize the microfluidic flow-flash method to probe the kinetics of CO recombination or O2 binding to myoglobin after the laser-induced photolysis of CO from myoglobin by UV/visible absorbance spectral imaging.
Authors:
Michael W Toepke; Scott H Brewer; Dung M Vu; Kirk D Rector; Joel E Morgan; Robert B Gennis; Paul J A Kenis; R Brian Dyer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  79     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-29     Completed Date:  2007-03-13     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carbon Monoxide / chemistry,  metabolism
Fluorescence
Kinetics
Lasers
Microfluidics / instrumentation,  methods*
Microscopy, Confocal / methods
Myoglobin / chemistry*,  metabolism
Oxygen / chemistry,  metabolism
Photolysis
Spectrometry, Fluorescence / methods
Spectrophotometry, Ultraviolet / methods
Grant Support
ID/Acronym/Agency:
GM 068036/GM/NIGMS NIH HHS; HL 16101/HL/NHLBI NIH HHS; R01 HL016101/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Myoglobin; 7U1EE4V452/Carbon Monoxide; S88TT14065/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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