Document Detail


Microfluidic fabrication of cell adhesive chitosan microtubes.
MedLine Citation:
PMID:  23355068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chitosan has been used as a scaffolding material in tissue engineering due to its mechanical properties and biocompatibility. With increased appreciation of the effect of micro- and nanoscale environments on cellular behavior, there is increased emphasis on generating microfabricated chitosan structures. Here we employed a microfluidic coaxial flow-focusing system to generate cell adhesive chitosan microtubes of controlled sizes by modifying the flow rates of a chitosan pre-polymer solution and phosphate buffered saline (PBS). The microtubes were extruded from a glass capillary with a 300 μm inner diameter. After ionic crosslinking with sodium tripolyphosphate (TPP), fabricated microtubes had inner and outer diameter ranges of 70-150 μm and 120-185 μm. Computational simulation validated the controlled size of microtubes and cell attachment. To enhance cell adhesiveness on the microtubes, we mixed gelatin with the chitosan pre-polymer solution. During the fabrication of microtubes, fibroblasts suspended in core PBS flow adhered to the inner surface of chitosan-gelatin microtubes. To achieve physiological pH values, we adjusted pH values of chiotsan pre-polymer solution and TPP. In particular, we were able to improve cell viability to 92 % with pH values of 5.8 and 7.4 for chitosan and TPP solution respectively. Cell culturing for three days showed that the addition of the gelatin enhanced cell spreading and proliferation inside the chitosan-gelatin microtubes. The microfluidic fabrication method for ionically crosslinked chitosan microtubes at physiological pH can be compatible with a variety of cells and used as a versatile platform for microengineered tissue engineering.
Authors:
Jonghyun Oh; Keekyoung Kim; Sung Wook Won; Chaenyung Cha; Akhilesh K Gaharwar; Seila Selimović; Hojae Bae; Kwang Ho Lee; Dong Hwan Lee; Sang-Hoon Lee; Ali Khademhosseini
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biomedical microdevices     Volume:  15     ISSN:  1572-8781     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-10     Completed Date:  2014-01-13     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  465-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion / drug effects
Cell Survival / drug effects
Chitosan / chemistry*,  pharmacology*
Computer Simulation
Gelatin / chemistry
Hydrogen-Ion Concentration
Materials Testing
Mechanical Processes
Mice
Microfluidic Analytical Techniques / instrumentation,  methods*
NIH 3T3 Cells
Polyphosphates / chemistry
Grant Support
ID/Acronym/Agency:
AR057837/AR/NIAMS NIH HHS; DE021468/DE/NIDCR NIH HHS; EB008392/EB/NIBIB NIH HHS; EB012597/EB/NIBIB NIH HHS; GM095906/GM/NIGMS NIH HHS; HL092836/HL/NHLBI NIH HHS; HL099073/HL/NHLBI NIH HHS; R01 AR057837/AR/NIAMS NIH HHS; R01 DE021468/DE/NIDCR NIH HHS; R01 EB008392/EB/NIBIB NIH HHS; R01 EB012597/EB/NIBIB NIH HHS; R01 GM095906/GM/NIGMS NIH HHS; R01 HL092836/HL/NHLBI NIH HHS; R01 HL099073/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Polyphosphates; 9000-70-8/Gelatin; 9012-76-4/Chitosan; NU43IAG5BC/triphosphoric acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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