Document Detail


Microfluidic device for dielectrophoresis manipulation and electrodisruption of respiratory pathogen Bordetella pertussis.
MedLine Citation:
PMID:  18838368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A miniaturized microfluidic device was developed to facilitate electromanipulation of bacterial respiratory pathogens. The device comprises a microchip with circular aluminum electrodes patterned on glass, which is housed in a microfluidic system fabricated utilizing polydimethylsiloxane. The device provides sample preparation capability by exploiting positive dielectrophoresis (DEP) in conjunction with pulsed voltage for manipulation and disruption of Bordetella pertussis bacterial cells. Positive DEP capture of B. pertussis was successfully demonstrated utilizing 10 Vrms and 1 MHz ac fields. Application of dc pulses (300 V amplitude and 50 micros pulsewidth applied 1 s apart) across the aluminum electrodes resulted in electrodisruption and lysis of B. pertussis bacterial cells. Real-time polymerase chain reaction, a 2(3) factorial experimental design and transmission electron microscopy were used to evaluate bacterial cell manipulation and factors affecting bacterial cell disruption. The main factors affecting bacterial cell disruption were electric field strength, the electrical conductivity of the cell suspension sample, and the combined effect of number of pulses and sample conductivity. The bacterial deoxyribonucleic acid target remained undamaged as a result of DEP and cell lysis experimentation. Our findings suggest that a simple miniaturized microfluidic device can achieve important steps in sample preparation on-chip involving respiratory bacterial pathogens.
Authors:
Carlos de la Rosa; Peter A Tilley; Julie D Fox; Karan V I S Kaler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  55     ISSN:  1558-2531     ISO Abbreviation:  IEEE Trans Biomed Eng     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-07     Completed Date:  2008-12-18     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2426-32     Citation Subset:  IM    
Affiliation:
Schulich School of Engineering, Department of Electrical and Computer Engineering, Calgary, AB T2N 1N4, Canada. cdelaro@ucalgary.ca
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MeSH Terms
Descriptor/Qualifier:
Bordetella pertussis / radiation effects*
Electric Conductivity
Electrophoresis, Microchip / instrumentation*,  methods
Electroporation / instrumentation*,  methods
Equipment Design / methods
Microelectrodes
Microfluidic Analytical Techniques*
Microfluidics / instrumentation,  methods
Research / instrumentation,  methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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