| Microfluidic Lysis of Human Blood for Leukocyte Analysis Using Single Cell Impedance Cytometry. | |
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MedLine Citation:
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PMID: 22148390 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. The microfluidic network design for RBC lysis is presented and the diffusive mixing processes analysed using experimental and simulated results. Healthy and clinical bloods were analyzed with this system and the data shows good correlation against data obtained from commercial haematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R2 (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes and 0.99 for granulocytes in terms of relative counts, and 0.94 for lymphocytes, 0.91 for monocytes and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose. |
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Authors:
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Xiaojun Han; Cees van Berkel; James D Gwyer; Lorenzo Carpetto; Hywel Morgan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-9 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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