Document Detail


Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis.
MedLine Citation:
PMID:  22897670     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers. Surface-marker-based detection of CTC predicts recurrence and survival in colorectal, breast, and prostate cancer. However, scarcity and variation in size, morphology, expression profile, and antigen exposure impairs reliable detection and characterization of CTC. We have developed a noncontact, label-free microfluidic acoustophoresis method to separate prostate cancer cells from white blood cells (WBC) through forces generated by ultrasonic resonances in microfluidic channels. Implementation of cell prealignment in a temperature-stabilized (±0.5 °C) acoustophoresis microchannel dramatically enhanced the discriminatory capacity and enabled the separation of 5 μm microspheres from 7 μm microspheres with 99% purity. Next, we determined the feasibility of employing label-free microfluidic acoustophoresis to discriminate and divert tumor cells from WBCs using erythrocyte-lysed blood from healthy volunteers spiked with tumor cells from three prostate cancer cell-lines (DU145, PC3, LNCaP). For cells fixed with paraformaldehyde, cancer cell recovery ranged from 93.6% to 97.9% with purity ranging from 97.4% to 98.4%. There was no detectable loss of cell viability or cell proliferation subsequent to the exposure of viable tumor cells to acoustophoresis. For nonfixed, viable cells, tumor cell recovery ranged from 72.5% to 93.9% with purity ranging from 79.6% to 99.7%. These data contribute proof-in-principle that label-free microfluidic acoustophoresis can be used to enrich both viable and fixed cancer cells from WBCs with very high recovery and purity.
Authors:
Per Augustsson; Cecilia Magnusson; Maria Nordin; Hans Lilja; Thomas Laurell
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-28
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-03-04     Revised Date:  2013-09-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7954-62     Citation Subset:  IM    
Affiliation:
Department of Measurement Technology, Lund University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD45 / metabolism
Antigens, Neoplasm / metabolism
Cell Adhesion Molecules / metabolism
Cell Line, Tumor
Cell Proliferation
Cell Separation*
Cell Survival
Humans
Leukocytes / cytology
Male
Microfluidic Analytical Techniques / instrumentation,  methods*
Neoplastic Cells, Circulating / metabolism*
Prostatic Neoplasms / blood*
Temperature
Grant Support
ID/Acronym/Agency:
1R01CA160816-01A1/CA/NCI NIH HHS; P50 CA092629/CA/NCI NIH HHS; P50-CA92629/CA/NCI NIH HHS; R01 CA160816/CA/NCI NIH HHS; R33 CA 127768-03/CA/NCI NIH HHS; R33 CA127768/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Cell Adhesion Molecules; 0/tumor-associated antigen GA733; EC 3.1.3.48/Antigens, CD45
Comments/Corrections

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