| Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis. | |
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MedLine Citation:
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PMID: 22897670 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers. Surface-marker-based detection of CTC predicts recurrence and survival in colorectal, breast, and prostate cancer. However, scarcity and variation in size, morphology, expression profile, and antigen exposure impairs reliable detection and characterization of CTC. We have developed a noncontact, label-free microfluidic acoustophoresis method to separate prostate cancer cells from white blood cells (WBC) through forces generated by ultrasonic resonances in microfluidic channels. Implementation of cell prealignment in a temperature-stabilized (±0.5 °C) acoustophoresis microchannel dramatically enhanced the discriminatory capacity and enabled the separation of 5 μm microspheres from 7 μm microspheres with 99% purity. Next, we determined the feasibility of employing label-free microfluidic acoustophoresis to discriminate and divert tumor cells from WBCs using erythrocyte-lysed blood from healthy volunteers spiked with tumor cells from three prostate cancer cell-lines (DU145, PC3, LNCaP). For cells fixed with paraformaldehyde, cancer cell recovery ranged from 93.6% to 97.9% with purity ranging from 97.4% to 98.4%. There was no detectable loss of cell viability or cell proliferation subsequent to the exposure of viable tumor cells to acoustophoresis. For nonfixed, viable cells, tumor cell recovery ranged from 72.5% to 93.9% with purity ranging from 79.6% to 99.7%. These data contribute proof-in-principle that label-free microfluidic acoustophoresis can be used to enrich both viable and fixed cancer cells from WBCs with very high recovery and purity. |
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Authors:
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Per Augustsson; Cecilia Magnusson; Maria Nordin; Hans Lilja; Thomas Laurell |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-28 |
Journal Detail:
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Title: Analytical chemistry Volume: 84 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-18 Completed Date: 2013-03-04 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7954-62 Citation Subset: IM |
Affiliation:
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Department of Measurement Technology, Lund University, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD45
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metabolism Antigens, Neoplasm / metabolism Cell Adhesion Molecules / metabolism Cell Line, Tumor Cell Proliferation Cell Separation* Cell Survival Humans Leukocytes / cytology Male Microfluidic Analytical Techniques / instrumentation, methods* Neoplastic Cells, Circulating / metabolism* Prostatic Neoplasms / blood* Temperature |
| Grant Support | |
ID/Acronym/Agency:
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1R01CA160816-01A1/CA/NCI NIH HHS; P50-CA92629/CA/NCI NIH HHS; R01 CA160816/CA/NCI NIH HHS; R33 CA 127768-03/CA/NCI NIH HHS; R33 CA127768/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/Cell Adhesion Molecules; 0/tumor-associated antigen GA733; EC 3.1.3.48/Antigens, CD45 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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