Document Detail


Microflora of soil as viewed by freeze-etching.
MedLine Citation:
PMID:  4123238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The indigenous microflora of soil were released from the soil materials and concentrated without the occurrence of growth by use of a blending-simple centrifugation procedure. The cell concentrate was then frozen-etched and viewed by transmission electron microscopy. Criteria were established for detecting microbial cells among the residual soil debris. The freeze-etching of the soil cell concentrate provided results on cell size distributions in agreement with those obtained by thin sectioning. However, the blending-simple centrifugation procedure for cell release and concentration from soil allowed the observation of large cells (>/=1.0 mum in diameter) which apparently are missed by the "exhaustive centrifugal washing" cell separation-concentration procedure. The procedure of blending-simple centrifugation combined with the viewing of frozenetched preparations allowed evaluations of the soil microflora for cellular diameters, length-width ratios, shapes, and structure.
Authors:
D L Balkwill; L E Casida
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  114     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1973 Jun 
Date Detail:
Created Date:  1973-08-21     Completed Date:  1973-08-21     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1319-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteria / cytology
Cell Membrane
Cell Wall
Centrifugation
Cytoplasm
Freeze Etching
Microscopy, Electron
Soil Microbiology*
Staining and Labeling
Comments/Corrections

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