| Microfabricated platforms for mechanically dynamic cell culture. | |
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MedLine Citation:
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PMID: 21206477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability to systematically probe in vitro cellular response to combinations of mechanobiological stimuli for tissue engineering, drug discovery or fundamental cell biology studies is limited by current bioreactor technologies, which cannot simultaneously apply a variety of mechanical stimuli to cultured cells. In order to address this issue, we have developed a series of microfabricated platforms designed to screen for the effects of mechanical stimuli in a high-throughput format. In this protocol, we demonstrate the fabrication of a microactuator array of vertically displaced posts on which the technology is based, and further demonstrate how this base technology can be modified to conduct high-throughput mechanically dynamic cell culture in both two-dimensional and three-dimensional culture paradigms. |
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Authors:
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Christopher Moraes; Yu Sun; Craig A Simmons |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media Date: 2010-12-26 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2010 |
Date Detail:
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Created Date: 2011-01-05 Completed Date: 2011-01-31 Revised Date: 2013-02-20 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Mechanical and Industrial Engineering, University of Toronto, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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instrumentation,
methods* Dimethylpolysiloxanes / chemistry Hydrogels / chemistry Microtechnology / instrumentation, methods* Polyethylene Glycols / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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CHRPJ 323533-06//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Dimethylpolysiloxanes; 0/Hydrogels; 0/Polyethylene Glycols; 63148-62-9/baysilon |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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