Document Detail


Microfabricated collagen tracks facilitate single cell metastatic invasion in 3D.
MedLine Citation:
PMID:  23388698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
While the mechanisms employed by metastatic cancer cells to migrate remain poorly understood, it has been widely accepted that metastatic cancer cells can invade the tumor stroma by degrading the extracellular matrix (ECM) with matrix metalloproteinases (MMPs). Although MMP inhibitors showed early promise in preventing metastasis in animal models, they have largely failed clinically. Recently, studies have shown that some cancer cells can use proteolysis to mechanically rearrange their ECM to form tube-like "microtracks" which other cells can follow without using MMPs themselves. We speculate that this mode of migration in the secondary cells may be one example of migration which can occur without endogenous protease activity in the secondary cells. Here we present a technique to study this migration in a 3D, collagen-based environment which mimics the size and topography of the tracks produced by proteolytically active cancer cells. Using time-lapse phase-contrast microscopy, we find that these microtracks permit the rapid and persistent migration of noninvasive MCF10A mammary epithelial cells, which are unable to otherwise migrate in 3D collagen. Additionally, while highly metastatic MDAMB231 breast cancer cells are able to invade a 3D collagen matrix, seeding within the patterned microtracks induced significantly increased cell migration speed, which was not decreased by pharmacological MMP inhibition. Together, these data suggest that microtracks within a 3D ECM may facilitate the migration of cells in an MMP-independent fashion, and may reveal novel insight into the clinical challenges facing MMP inhibitors.
Authors:
Casey M Kraning-Rush; Shawn P Carey; Marsha C Lampi; Cynthia A Reinhart-King
Related Documents :
24516828 - Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on...
22829218 - Giant vesicles as cell models.
24871928 - Dynamics and evolution of β-catenin-dependent wnt signaling revealed through massively...
22811958 - Local isotropic phase symmetry measure for detection of beta cells and lymphocytes.
7530908 - Differential expression of orcc and cftr induced by low temperature in cf airway epithe...
187268 - Thymidine kinase gene transfer by herpes simplex virus.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  5     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-25     Completed Date:  2013-09-24     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  606-16     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / metabolism
Cell Culture Techniques*
Cell Line, Tumor
Cell Movement
Collagen / chemistry*
Culture Media
Extracellular Matrix / metabolism*
Female
Fluorescent Dyes
Humans
Matrix Metalloproteinases / metabolism*
Microscopy, Phase-Contrast
Models, Biological
Neoplasm Invasiveness
Neoplasm Metastasis
RNA Interference
Grant Support
ID/Acronym/Agency:
U54 CA143876/CA/NCI NIH HHS; U54CA143876/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fluorescent Dyes; 9007-34-5/Collagen; EC 3.4.24.-/Matrix Metalloproteinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The cytospin technique improves the detection of calcium pyrophosphate crystals in synovial fluid sa...
Next Document:  High pressure luminescence spectra of CaMoO(4):Ln(3+) (Ln = Pr, Tb).