Document Detail

Microfabricated collagen tracks facilitate single cell metastatic invasion in 3D.
MedLine Citation:
PMID:  23388698     Owner:  NLM     Status:  MEDLINE    
While the mechanisms employed by metastatic cancer cells to migrate remain poorly understood, it has been widely accepted that metastatic cancer cells can invade the tumor stroma by degrading the extracellular matrix (ECM) with matrix metalloproteinases (MMPs). Although MMP inhibitors showed early promise in preventing metastasis in animal models, they have largely failed clinically. Recently, studies have shown that some cancer cells can use proteolysis to mechanically rearrange their ECM to form tube-like "microtracks" which other cells can follow without using MMPs themselves. We speculate that this mode of migration in the secondary cells may be one example of migration which can occur without endogenous protease activity in the secondary cells. Here we present a technique to study this migration in a 3D, collagen-based environment which mimics the size and topography of the tracks produced by proteolytically active cancer cells. Using time-lapse phase-contrast microscopy, we find that these microtracks permit the rapid and persistent migration of noninvasive MCF10A mammary epithelial cells, which are unable to otherwise migrate in 3D collagen. Additionally, while highly metastatic MDAMB231 breast cancer cells are able to invade a 3D collagen matrix, seeding within the patterned microtracks induced significantly increased cell migration speed, which was not decreased by pharmacological MMP inhibition. Together, these data suggest that microtracks within a 3D ECM may facilitate the migration of cells in an MMP-independent fashion, and may reveal novel insight into the clinical challenges facing MMP inhibitors.
Casey M Kraning-Rush; Shawn P Carey; Marsha C Lampi; Cynthia A Reinhart-King
Related Documents :
23634818 - The role of n-doped multi-wall carbon nanotubes in achieving highly efficient polymer b...
23097598 - The plasminogen activation system and the regulation of catecholaminergic function.
23894488 - Japanese encephalitis virus disrupts cell-cell junctions and affects the epithelial per...
23460808 - Estrogen inhibits renal cell carcinoma cell progression through estrogen receptor-β ac...
11443038 - Functional modeling of tight junctions in intestinal cell monolayers using polyethylene...
21596708 - Establishment and characterization of a spontaneously immortalized trophoblast cell lin...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  5     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-25     Completed Date:  2013-09-24     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  606-16     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Breast Neoplasms / metabolism
Cell Culture Techniques*
Cell Line, Tumor
Cell Movement
Collagen / chemistry*
Culture Media
Extracellular Matrix / metabolism*
Fluorescent Dyes
Matrix Metalloproteinases / metabolism*
Microscopy, Phase-Contrast
Models, Biological
Neoplasm Invasiveness
Neoplasm Metastasis
RNA Interference
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Fluorescent Dyes; 9007-34-5/Collagen; EC 3.4.24.-/Matrix Metalloproteinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The cytospin technique improves the detection of calcium pyrophosphate crystals in synovial fluid sa...
Next Document:  High pressure luminescence spectra of CaMoO(4):Ln(3+) (Ln = Pr, Tb).