Document Detail


Microengineered surface topography facilitates cell grafting from a prototype hydrogel wound dressing with antibacterial capability.
MedLine Citation:
PMID:  17022681     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skin wounds derive therapeutic benefit from redeployment of dermal tissues, whether as split-thickness allo- and autografts or as biological dressings comprising cultured cells. However, the clinical outcome is strongly influenced by the techniques used for cell/tissue grafting and also the microbiological status of the wound. Here we report that microtopography incorporated into the surface of a novel polymeric material, derivatized with fibronectin to promote attachment and encourage motility, improved the efficiency of cell transfer onto de-epithelialized human skin ex vivo. The microtopography had two functions, first as a conduit for migrating cells to cross between the vehicle and recipient surface and second to shield adherent cells from destruction by mechanical shearing during handling and application. Quantitative analysis showed that topographic projections (columns) rather than recesses (pits) in the hydrogel surface achieved the highest efficiency of cell transfer. In order to address the crucial relevance of microbiological contamination to the success of wound grafting, the effect of iodine on several common bacterial pathogens was examined using an XTT+C(Q10) kinetic cell viability assay. Increasing concentrations of iodine initially stressed and after 0.5% v/v were subsequently bacteriocidal for Gram-negative Pseudomonas aeruginosa and Escherichia coli and Gram-positive Bacillus subtillis and Staphylococcus aureus. Slightly higher doses of iodine (approx 1-1.5% v/v) were required to kill HaCaT cells outright, but for both pro- and eukaryotes the major determinant of cytotoxicity was absolute dose rather than duration of exposure. Iodine delivered by the hydrogel at low concentration was bacteriostatic but not apparently cytotoxic to epithelial cells as measured by MTT end-point cell viability assay. Zone of inhibition studies confirmed that bacteriocidal quantities of neomycin, phenol red, and silver could also be delivered using the same hydrogel. This research suggests that grafting cell-based biological dressings to wounds using a topographically modified hydrogel dressing capable of simultaneous reducing the microbiological threat to a successful outcome may be a realistic clinical proposition.
Authors:
Annie G Smith; Abbas Din; Morgan Denyer; Nicholas J Crowther; Donald Eagland; Kath Vowden; Peter Vowden; Stephen T Britland
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  22     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2006 Sep-Oct
Date Detail:
Created Date:  2006-10-06     Completed Date:  2007-01-17     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1407-15     Citation Subset:  IM    
Affiliation:
School of Pharmacy, University of Bradford, UK, AGT Sciences Ltd., Listerhills Science Park, Bradford, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents / pharmacology*
Bacillus subtilis / metabolism
Bandages
Escherichia coli / metabolism
Fibroblasts / microbiology
Humans
Hydrogel / chemistry*
Middle Aged
Pseudomonas aeruginosa / metabolism
Silicon / chemistry
Staphylococcus aureus / metabolism
Stress, Mechanical
Wound Healing
Wound Infection / prevention & control*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 25852-47-5/Hydrogel; 7440-21-3/Silicon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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