Document Detail


Microencapsulation of bacteriophage felix O1 into chitosan-alginate microspheres for oral delivery.
MedLine Citation:
PMID:  18515488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper reports the development of microencapsulated bacteriophage Felix O1 for oral delivery using a chitosan-alginate-CaCl(2) system. In vitro studies were used to determine the effects of simulated gastric fluid (SGF) and bile salts on the viability of free and encapsulated phage. Free phage Felix O1 was found to be extremely sensitive to acidic environments and was not detectable after a 5-min exposure to pHs below 3.7. In contrast, the number of microencapsulated phage decreased by 0.67 log units only, even at pH 2.4, for the same period of incubation. The viable count of microencapsulated phage decreased only 2.58 log units during a 1-h exposure to SGF with pepsin at pH 2.4. After 3 h of incubation in 1 and 2% bile solutions, the free phage count decreased by 1.29 and 1.67 log units, respectively, while the viability of encapsulated phage was fully maintained. Encapsulated phage was completely released from the microspheres upon exposure to simulated intestinal fluid (pH 6.8) within 6 h. The encapsulated phage in wet microspheres retained full viability when stored at 4 degrees C for the duration of the testing period (6 weeks). With the use of trehalose as a stabilizing agent, the microencapsulated phage in dried form had a 12.6% survival rate after storage for 6 weeks. The current encapsulation technique enables a large proportion of bacteriophage Felix O1 to remain bioactive in a simulated gastrointestinal tract environment, which indicates that these microspheres may facilitate delivery of therapeutic phage to the gut.
Authors:
Yongsheng Ma; Jennifer C Pacan; Qi Wang; Yongping Xu; Xiaoqing Huang; Anton Korenevsky; Parviz M Sabour
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-30
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-31     Completed Date:  2008-09-24     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4799-805     Citation Subset:  IM    
Affiliation:
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China.
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MeSH Terms
Descriptor/Qualifier:
Alginates*
Bacteriophages / drug effects,  isolation & purification*,  physiology*,  ultrastructure
Chitosan*
Drug Compounding / methods*
Gastric Juice
Glucuronic Acid
Hexuronic Acids
Hydrogen-Ion Concentration
Pepsin A / pharmacology
Salmonella typhi / virology
Chemical
Reg. No./Substance:
0/Alginates; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; 9012-76-4/Chitosan; EC 3.4.23.1/Pepsin A
Comments/Corrections

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