Document Detail

Microencapsulated bovine chromaffin cell xenografts into hemiparkinsonian rats: a drug-induced rotational behavior and histological changes analysis.
MedLine Citation:
PMID:  11251478     Owner:  NLM     Status:  MEDLINE    
Bovine chromaffin cells were microencapsulated within alginate-polylysine-alginate (APA) membranes. Microencapsulated bovine chromaffin cells as well as unencapsulated cells and empty microcapsules were grafted into the brain of hemiparkinsonian rats with 6-hydroxydopamine (6-OHDA) lesions. Apomorphine-induced rotational behavior of the host animals and the survival of the grafted chromaffin cells were examined after transplantation. The animals receiving microencapsulated bovine chromaffin cells showed a significant decrease (17.6--35.6%) in apomorphine-induced rotation 1 week postimplantation that remained stable for the 10 month test period. Fluorescent histochemistry further revealed that microencapsulation increased the chromaffin cell survival with only a minimum host reaction for up to 10 months posttransplantation while the survival of free, unencapsulated chromaffin cells was only modest and was accompanied by a large inflammatory response. The reduction of apomorphine-induced rotations was correlated with the survival of bovine chromaffin cells in the host brain. The data indicate that encapsulation of bovine chromaffin cells in APA membranes reduces the host immune response to the xenograft and prolongs the viability of the grafted cells.
Y Xue; J Gao; Z Xi; Z Wang; X Li; X Cui; Y Luo; C Li; L Wang; D Zhou; R Sun; A M Sun
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial organs     Volume:  25     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-19     Completed Date:  2001-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-5     Citation Subset:  IM    
Institute of Basic Medical Sciences, People's Liberation Army General Hospital, Beijing, China.
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MeSH Terms
Cell Survival
Chromaffin Cells / pathology,  transplantation*
Disease Models, Animal
Microscopy, Fluorescence
Parkinson Disease / therapy*
Parkinson Disease, Secondary / chemically induced
Psychomotor Disorders / chemically induced,  prevention & control*
Rats, Sprague-Dawley
Sensitivity and Specificity
Transplantation, Heterologous / methods
Reg. No./Substance:
0/Capsules; 58-00-4/Apomorphine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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