Document Detail


Microelectrode biosensor for real-time measurement of ATP in biological tissue.
MedLine Citation:
PMID:  15889918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purines ATP, ADP, and adenosine are important extracellular signaling agents. Analysis of purinergic signaling has been slowed by lack of direct methods for measurement of purine release in real-time during physiological activity. We have previously reported microelectrode biosensors for adenosine, but similar sensors for ATP have remained elusive. We now describe an ATP biosensor formed by coating a Pt microelectrode with an ultrathin biolayer containing glycerol kinase and glycerol-3-phosphate oxidase. It responds rapidly (10-90% rise time <10 s) and exhibits a linear response to ATP over the physiologically relevant concentrations of 200 nM-50 microM and is very sensitive approximately 250 mA.M(-1).cm(-2). By including phosphocreatine kinase in the biolayer, we can optionally amplify the ATP signal and also make the sensor sensitive to external ADP. We have used our sensors to make the first demonstration that ATP is released from spinal networks in vivo during locomotor activity.
Authors:
Enrique Llaudet; Sonja Hatz; Magali Droniou; Nicholas Dale
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  77     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-13     Completed Date:  2007-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3267-73     Citation Subset:  IM    
Affiliation:
Warwick Biosensors Group, Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / analysis*
Biosensing Techniques / instrumentation,  methods*
Creatine Kinase / metabolism
Electrochemistry / instrumentation,  methods
Extracellular Fluid / chemistry*
Glycerol Kinase / metabolism
Glycerolphosphate Dehydrogenase / metabolism
Hydrogen-Ion Concentration
Microelectrodes*
Platinum / chemistry*
Sensitivity and Specificity
Spinal Cord / physiology
Time Factors
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 7440-06-4/Platinum; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 1.1.3.21/glycerol-3-phosphate oxidase; EC 2.7.1.30/Glycerol Kinase; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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