| Microelectrode biosensor for real-time measurement of ATP in biological tissue. | |
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MedLine Citation:
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PMID: 15889918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purines ATP, ADP, and adenosine are important extracellular signaling agents. Analysis of purinergic signaling has been slowed by lack of direct methods for measurement of purine release in real-time during physiological activity. We have previously reported microelectrode biosensors for adenosine, but similar sensors for ATP have remained elusive. We now describe an ATP biosensor formed by coating a Pt microelectrode with an ultrathin biolayer containing glycerol kinase and glycerol-3-phosphate oxidase. It responds rapidly (10-90% rise time <10 s) and exhibits a linear response to ATP over the physiologically relevant concentrations of 200 nM-50 microM and is very sensitive approximately 250 mA.M(-1).cm(-2). By including phosphocreatine kinase in the biolayer, we can optionally amplify the ATP signal and also make the sensor sensitive to external ADP. We have used our sensors to make the first demonstration that ATP is released from spinal networks in vivo during locomotor activity. |
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Authors:
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Enrique Llaudet; Sonja Hatz; Magali Droniou; Nicholas Dale |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 77 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-05-13 Completed Date: 2007-03-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 3267-73 Citation Subset: IM |
Affiliation:
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Warwick Biosensors Group, Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Triphosphate / analysis* Biosensing Techniques / instrumentation, methods* Creatine Kinase / metabolism Electrochemistry / instrumentation, methods Extracellular Fluid / chemistry* Glycerol Kinase / metabolism Glycerolphosphate Dehydrogenase / metabolism Hydrogen-Ion Concentration Microelectrodes* Platinum / chemistry* Sensitivity and Specificity Spinal Cord / physiology Time Factors |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 7440-06-4/Platinum; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 1.1.3.21/glycerol-3-phosphate oxidase; EC 2.7.1.30/Glycerol Kinase; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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